Alteration of the Circadian Clock in Children with Smith-Magenis Syndrome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A11578" target="_blank" >RIV/00216208:11110/12:11578 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/12:00376807 RIV/00064165:_____/12:11578

Výsledek na webu

<a href="http://dx.doi.org/10.1210/jc.2011-2750" target="_blank" >http://dx.doi.org/10.1210/jc.2011-2750</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Alteration of the Circadian Clock in Children with Smith-Magenis Syndrome

Popis výsledku v původním jazyce

Context: Smith-Magenis syndrome (SMS) is associated with sleep disturbances and disrupted melatonin production. Objectives: The study aimed to ascertain whether the sleep and melatonin production anomalies in SMS patients may be due to an alteration of the molecular mechanism of the circadian clock. Subjects and Methods: Five SMS patients (3-17 yr old) and five healthy age-matched control subjects were involved in the study. Saliva and buccal scrub samples were collected every 4 h during a 24-h period.Daily profiles of melatonin were determined in saliva using a direct double-antibody radioimmunoassay. Daily profiles of clock gene mRNA levels (Per1, Per2, and Rev-erb alpha) were determined in buccal scrub samples by RT-PCR. Results: In controls, melatonin levels were elevated during the nighttime and very low during the daytime. Daily profiles of clock genes, Per1, Per2, and Rev-erb alpha, mRNA levels in buccal mucosa exhibited significant and mutually synchronized circadian variation

Název v anglickém jazyce

Alteration of the Circadian Clock in Children with Smith-Magenis Syndrome

Popis výsledku anglicky

Context: Smith-Magenis syndrome (SMS) is associated with sleep disturbances and disrupted melatonin production. Objectives: The study aimed to ascertain whether the sleep and melatonin production anomalies in SMS patients may be due to an alteration of the molecular mechanism of the circadian clock. Subjects and Methods: Five SMS patients (3-17 yr old) and five healthy age-matched control subjects were involved in the study. Saliva and buccal scrub samples were collected every 4 h during a 24-h period.Daily profiles of melatonin were determined in saliva using a direct double-antibody radioimmunoassay. Daily profiles of clock gene mRNA levels (Per1, Per2, and Rev-erb alpha) were determined in buccal scrub samples by RT-PCR. Results: In controls, melatonin levels were elevated during the nighttime and very low during the daytime. Daily profiles of clock genes, Per1, Per2, and Rev-erb alpha, mRNA levels in buccal mucosa exhibited significant and mutually synchronized circadian variation

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/NT11474" target="_blank" >NT11474: Určení funkčního stavu cirkadiánního systému člověka a jeho změny u depresivní a bipolární poruchy</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Clinical Endocrinology and Metabolism

ISSN

0021-972X

e-ISSN

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Svazek periodika

97

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

"E312"-"E318"

Kód UT WoS článku

000301226800021

EID výsledku v databázi Scopus

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