Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189736" target="_blank" >RIV/00216208:11110/13:10189736 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/13:00399949
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s12223-012-0181-y" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0181-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-012-0181-y" target="_blank" >10.1007/s12223-012-0181-y</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Popis výsledku v původním jazyce
Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localizationof B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic c
Název v anglickém jazyce
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Popis výsledku anglicky
Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localizationof B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic c
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA310%2F07%2F0675" target="_blank" >GA310/07/0675: Slizniční imunizace chřipkovým virem typu A</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Folia Microbiologica
ISSN
0015-5632
e-ISSN
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Svazek periodika
58
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
11
Strana od-do
111-121
Kód UT WoS článku
000314409400003
EID výsledku v databázi Scopus
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