Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10192269" target="_blank" >RIV/00216208:11110/13:10192269 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/liv.12219" target="_blank" >http://dx.doi.org/10.1111/liv.12219</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/liv.12219" target="_blank" >10.1111/liv.12219</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups

Popis výsledku v původním jazyce

Background Free bilirubin (Bf), the unbound fraction of unconjugated bilirubin (UCB), can induce neurotoxicity, including impairment of the auditory system, which can be assessed by brainstem auditory evoked potentials (BAEPs). We hypothesized that albumin might reduce the risk of neurotoxicity by decreasing Bf and its translocation into the brain. AimTo determine the effects of albumin on BAEPs and brain bilirubin content in two Gunn rat pup models of acute hyperbilirubinemia. MethodsWe used Gunn rat pups, which have a deficiency of the bilirubin-conjugating enzyme UGT1A1. We induced haemolysis by injection of phenylhydrazine (phz) into 14-days old pups. Subsequently, pups were treated with either i.p. human serum albumin (HSA; 2.5g/kg; n=8) or saline(control, n=8). We induced acute neurotoxicity by injecting 16-days old pups with sulphadimethoxine (sulpha) and treated them with either HSA (n=9) or saline (control, n=10). To assess bilirubin neurotoxicity, we used the validated BAEP

Název v anglickém jazyce

Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups

Popis výsledku anglicky

Background Free bilirubin (Bf), the unbound fraction of unconjugated bilirubin (UCB), can induce neurotoxicity, including impairment of the auditory system, which can be assessed by brainstem auditory evoked potentials (BAEPs). We hypothesized that albumin might reduce the risk of neurotoxicity by decreasing Bf and its translocation into the brain. AimTo determine the effects of albumin on BAEPs and brain bilirubin content in two Gunn rat pup models of acute hyperbilirubinemia. MethodsWe used Gunn rat pups, which have a deficiency of the bilirubin-conjugating enzyme UGT1A1. We induced haemolysis by injection of phenylhydrazine (phz) into 14-days old pups. Subsequently, pups were treated with either i.p. human serum albumin (HSA; 2.5g/kg; n=8) or saline(control, n=8). We induced acute neurotoxicity by injecting 16-days old pups with sulphadimethoxine (sulpha) and treated them with either HSA (n=9) or saline (control, n=10). To assess bilirubin neurotoxicity, we used the validated BAEP

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Liver International

ISSN

1478-3223

e-ISSN

—

Svazek periodika

33

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1557-1565

Kód UT WoS článku

000325362200014

EID výsledku v databázi Scopus

—