The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS- Production and Evidence of Pancreatic and Lung Toxicity
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10193137" target="_blank" >RIV/00216208:11110/13:10193137 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00064165:_____/13:10193137
Výsledek na webu
<a href="http://dx.doi.org/10.1089/ars.2012.5010" target="_blank" >http://dx.doi.org/10.1089/ars.2012.5010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/ars.2012.5010" target="_blank" >10.1089/ars.2012.5010</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS- Production and Evidence of Pancreatic and Lung Toxicity
Popis výsledku v původním jazyce
Aims: To define the consequences of loss of cysteine dioxygenase (CDO) on cysteine metabolism at the tissue level, we determined levels of relevant metabolites and enzymes and evidence of H2S/HS- (gaseous hydrogen sulfide and its conjugate base) toxicityin liver, pancreas, kidney, and lung of CDO-/- mice that were fed either a taurine-free or taurine-supplemented diet. Results: CDO-/- mice had low tissue and serum taurine and hypotaurine levels and high tissue levels of cysteine, consistent with the loss of CDO. CDO-/- mice had elevated urinary excretion of thiosulfate, high tissue and serum cystathionine and lanthionine levels, and evidence of inhibition and destabilization of cytochrome c oxidase, which is consistent with excess production of H2S/HS-. Accumulation of cystathionine and lanthionine appeared to result from cystathionine -synthase (CBS)-mediated cysteine desulfhydration. Very high levels of hypotaurine in pancreas of wild-type mice and very high levels of cystathionine
Název v anglickém jazyce
The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS- Production and Evidence of Pancreatic and Lung Toxicity
Popis výsledku anglicky
Aims: To define the consequences of loss of cysteine dioxygenase (CDO) on cysteine metabolism at the tissue level, we determined levels of relevant metabolites and enzymes and evidence of H2S/HS- (gaseous hydrogen sulfide and its conjugate base) toxicityin liver, pancreas, kidney, and lung of CDO-/- mice that were fed either a taurine-free or taurine-supplemented diet. Results: CDO-/- mice had low tissue and serum taurine and hypotaurine levels and high tissue levels of cysteine, consistent with the loss of CDO. CDO-/- mice had elevated urinary excretion of thiosulfate, high tissue and serum cystathionine and lanthionine levels, and evidence of inhibition and destabilization of cytochrome c oxidase, which is consistent with excess production of H2S/HS-. Accumulation of cystathionine and lanthionine appeared to result from cystathionine -synthase (CBS)-mediated cysteine desulfhydration. Very high levels of hypotaurine in pancreas of wild-type mice and very high levels of cystathionine
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Antioxidants and Redox Signaling
ISSN
1523-0864
e-ISSN
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Svazek periodika
19
Číslo periodika v rámci svazku
12
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
16
Strana od-do
1321-1336
Kód UT WoS článku
000325267800002
EID výsledku v databázi Scopus
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