The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10195513" target="_blank" >RIV/00216208:11110/13:10195513 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fitote.2013.03.016" target="_blank" >http://dx.doi.org/10.1016/j.fitote.2013.03.016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fitote.2013.03.016" target="_blank" >10.1016/j.fitote.2013.03.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats

Popis výsledku v původním jazyce

The objective of this study was to evaluate potential hepatoprotective capabilities of quercetin in relation to its modulation of the HO-1 and NOS-2 activities in an experimental model of fulminant liver failure. Liver insult was induced by in vivo administration of D-galactosamine (D-GaIN, 400 mg/kg, i.p.) and lipopolysaccharide (LPS, 10 mu g/kg, i.p.). The effects of quercetin (50 mg/kg, i.p) on D-GaIN toxicity was evaluated by standard biochemical, RT-PCR and Western blot methods. Administration of D-GaIN/LPS combination resulted in significantly higher plasma levels of aminotransferases, as well as increased mRNA and protein expressions of both HO-1 and NOS-2 enzymes. Quercetin exhibited cytoprotective effects on the liver, as evidenced by decreased aminotransferase plasma levels. Additionally, quercetin treatment in D-GaIN/LPS treated rats significantly increased HO-1 mRNA and its protein expressions. On the contrary, quercetin did not exhibit any significant effects on the levels

Název v anglickém jazyce

The involvement of heme oxygenase 1 but not nitric oxide synthase 2 in a hepatoprotective action of quercetin in lipopolysaccharide-induced hepatotoxicity of D-galactosamine sensitized rats

Popis výsledku anglicky

The objective of this study was to evaluate potential hepatoprotective capabilities of quercetin in relation to its modulation of the HO-1 and NOS-2 activities in an experimental model of fulminant liver failure. Liver insult was induced by in vivo administration of D-galactosamine (D-GaIN, 400 mg/kg, i.p.) and lipopolysaccharide (LPS, 10 mu g/kg, i.p.). The effects of quercetin (50 mg/kg, i.p) on D-GaIN toxicity was evaluated by standard biochemical, RT-PCR and Western blot methods. Administration of D-GaIN/LPS combination resulted in significantly higher plasma levels of aminotransferases, as well as increased mRNA and protein expressions of both HO-1 and NOS-2 enzymes. Quercetin exhibited cytoprotective effects on the liver, as evidenced by decreased aminotransferase plasma levels. Additionally, quercetin treatment in D-GaIN/LPS treated rats significantly increased HO-1 mRNA and its protein expressions. On the contrary, quercetin did not exhibit any significant effects on the levels

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fitoterapia

ISSN

0367-326X

e-ISSN

—

Svazek periodika

87

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

20-26

Kód UT WoS článku

000319953100004

EID výsledku v databázi Scopus

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