Different cytokine production and toll-like receptor expression induced by heat-killed invasive and carrier strains of Neisseria meningitidis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10194775" target="_blank" >RIV/00216208:11110/14:10194775 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/apm.12062" target="_blank" >http://dx.doi.org/10.1111/apm.12062</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/apm.12062" target="_blank" >10.1111/apm.12062</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Different cytokine production and toll-like receptor expression induced by heat-killed invasive and carrier strains of Neisseria meningitidis

Popis výsledku v původním jazyce

Neisseria meningitidis may cause severe invasive disease. The carriage state of the pathogen is common, and the reasons underlying why the infection becomes invasive are not fully understood. The aim of this study was to compare the differences between invasive and carrier strains in the activation of innate immunity. The monocyte expression of TLR2, TLR4, CD14, and HLA-DR, cytokine production, and the granulocyte oxidative burst were analyzed after in vitro stimulation by heat-killed invasive (n=14) and carrier (n=9) strains of N. meningitidis. The expression of the cell surface markers in monocytes, the oxidative burst, and cytokine concentrations were measured using flow cytometry. Carrier strains stimulated a higher production of inflammatory cytokines and oxidative burst in granulocytes than invasive strains (all p<0.001), whereas invasive strains significantly up-regulated TLR2, TLR4 (p<0.001), and CD14 (p<0.01) expression on monocytes. Conversely, the monocyte expression of HLA-

Název v anglickém jazyce

Different cytokine production and toll-like receptor expression induced by heat-killed invasive and carrier strains of Neisseria meningitidis

Popis výsledku anglicky

Neisseria meningitidis may cause severe invasive disease. The carriage state of the pathogen is common, and the reasons underlying why the infection becomes invasive are not fully understood. The aim of this study was to compare the differences between invasive and carrier strains in the activation of innate immunity. The monocyte expression of TLR2, TLR4, CD14, and HLA-DR, cytokine production, and the granulocyte oxidative burst were analyzed after in vitro stimulation by heat-killed invasive (n=14) and carrier (n=9) strains of N. meningitidis. The expression of the cell surface markers in monocytes, the oxidative burst, and cytokine concentrations were measured using flow cytometry. Carrier strains stimulated a higher production of inflammatory cytokines and oxidative burst in granulocytes than invasive strains (all p<0.001), whereas invasive strains significantly up-regulated TLR2, TLR4 (p<0.001), and CD14 (p<0.01) expression on monocytes. Conversely, the monocyte expression of HLA-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

ISSN

0903-4641

e-ISSN

—

Svazek periodika

122

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

9

Strana od-do

33-41

Kód UT WoS článku

000328909100005

EID výsledku v databázi Scopus

—