The effect of protein corona composition on the interaction of carbon nanotubes with human blood platelets

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10272441" target="_blank" >RIV/00216208:11110/14:10272441 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biomaterials.2014.04.067" target="_blank" >http://dx.doi.org/10.1016/j.biomaterials.2014.04.067</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biomaterials.2014.04.067" target="_blank" >10.1016/j.biomaterials.2014.04.067</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of protein corona composition on the interaction of carbon nanotubes with human blood platelets

Popis výsledku v původním jazyce

Carbon nanotubes (CNT) are one of the most promising nanomaterials for use in medicine. The blood biocompatibility of CNT is a critical safety issue. In the bloodstream, proteins bind to CNT through non-covalent interactions to form a protein corona, thereby largely defining the biological properties of the CNT. Here, we characterize the interactions of carboxylated-multiwalled carbon nanotubes (CNTCOOH) with common human proteins and investigate the effect of the different protein coronas on the interaction of CNTCOOH with human blood platelets (PLT). Molecular modeling and different photophysical techniques were employed to characterize the binding of albumin (HSA), fibrinogen (FBG), gamma-globulins (IgG) and histone H1 (H1) on CNTCOOH. We found thatthe identity of protein forming the corona greatly affects the outcome of CNTCOOH's interaction with blood PLT. Bare CNTCOOH-induced PLT aggregation and the release of platelet membrane microparticles (PMP). HSA corona attenuated the PLT

Název v anglickém jazyce

The effect of protein corona composition on the interaction of carbon nanotubes with human blood platelets

Popis výsledku anglicky

Carbon nanotubes (CNT) are one of the most promising nanomaterials for use in medicine. The blood biocompatibility of CNT is a critical safety issue. In the bloodstream, proteins bind to CNT through non-covalent interactions to form a protein corona, thereby largely defining the biological properties of the CNT. Here, we characterize the interactions of carboxylated-multiwalled carbon nanotubes (CNTCOOH) with common human proteins and investigate the effect of the different protein coronas on the interaction of CNTCOOH with human blood platelets (PLT). Molecular modeling and different photophysical techniques were employed to characterize the binding of albumin (HSA), fibrinogen (FBG), gamma-globulins (IgG) and histone H1 (H1) on CNTCOOH. We found thatthe identity of protein forming the corona greatly affects the outcome of CNTCOOH's interaction with blood PLT. Bare CNTCOOH-induced PLT aggregation and the release of platelet membrane microparticles (PMP). HSA corona attenuated the PLT

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/LH12014" target="_blank" >LH12014: Analýza buněčné toxicity nanočástic a proteinových amyloidových fibril</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomaterials

ISSN

0142-9612

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

6182-6194

Kód UT WoS článku

000338804500005

EID výsledku v databázi Scopus

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