Tracking DNA and RNA Sequences at High Resolution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10284235" target="_blank" >RIV/00216208:11110/14:10284235 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/14:33149229

Výsledek na webu

<a href="http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-776-1_16" target="_blank" >10.1007/978-1-62703-776-1_16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tracking DNA and RNA Sequences at High Resolution

Popis výsledku v původním jazyce

In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol forthe post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.

Název v anglickém jazyce

Tracking DNA and RNA Sequences at High Resolution

Popis výsledku anglicky

In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol forthe post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.

Druh

C - Kapitola v odborné knize

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Electron Microscopy; Methods and Protocols : Methods and Protocols

ISBN

978-1-62703-775-4

Počet stran výsledku

24

Strana od-do

343-366

Počet stran knihy

799

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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