Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285510" target="_blank" >RIV/00216208:11110/14:10285510 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1369/0022155414543853" target="_blank" >http://dx.doi.org/10.1369/0022155414543853</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1369/0022155414543853" target="_blank" >10.1369/0022155414543853</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions

Popis výsledku v původním jazyce

Inosine-5-monophosphate dehydrogenase catalyzes the critical step in the de novo synthesis of guanosine nucleotides: the oxidation of inosine monophosphate to xanthosine monophosphate. This reaction can be inhibited by specific inhibitors, such as ribavirin or mycophenolic acid, which are widely used in clinical treatment when required to inhibit the proliferation of viruses or cells. However, it was recently found that such an inhibition affects the cells, leading to a redistribution of IMPDH2 and theappearance of IMPDH2 inclusions in the cytoplasm. According to their shape, these inclusions have been termed Rods and Rings (R&R). In this work, we focused on the subcellular localization of IMPDH2 protein and the ultrastructure of R&R inclusions. Usingmicroscopy and western blot analysis, we show the presence of nuclear IMPDH2 in human cells. We also show that the nuclear pool has an ability to form Rod structures after inhibition by ribavirin. Concerning the ultrastructure, we observ

Název v anglickém jazyce

Ultrastructure of Cytoplasmic and Nuclear Inosine-5 '-Monophosphate Dehydrogenase 2 "Rods and Rings" Inclusions

Popis výsledku anglicky

Inosine-5-monophosphate dehydrogenase catalyzes the critical step in the de novo synthesis of guanosine nucleotides: the oxidation of inosine monophosphate to xanthosine monophosphate. This reaction can be inhibited by specific inhibitors, such as ribavirin or mycophenolic acid, which are widely used in clinical treatment when required to inhibit the proliferation of viruses or cells. However, it was recently found that such an inhibition affects the cells, leading to a redistribution of IMPDH2 and theappearance of IMPDH2 inclusions in the cytoplasm. According to their shape, these inclusions have been termed Rods and Rings (R&R). In this work, we focused on the subcellular localization of IMPDH2 protein and the ultrastructure of R&R inclusions. Usingmicroscopy and western blot analysis, we show the presence of nuclear IMPDH2 in human cells. We also show that the nuclear pool has an ability to form Rod structures after inhibition by ribavirin. Concerning the ultrastructure, we observ

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Histochemistry and Cytochemistry

ISSN

0022-1554

e-ISSN

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Svazek periodika

62

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

739-750

Kód UT WoS článku

000342836200004

EID výsledku v databázi Scopus

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