Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285970" target="_blank" >RIV/00216208:11110/14:10285970 - isvavai.cz</a>

Výsledek na webu

<a href="http://fb.cuni.cz/file/5718/FB2014A0010.pdf" target="_blank" >http://fb.cuni.cz/file/5718/FB2014A0010.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers

Popis výsledku v původním jazyce

The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The maturation state (presence of CD83) of cord blood monocyte-derived dendritic cells (moDCs) 01 52 children of healthy mothers and 58 children of allergic mothers was estimated by flow cytometry. The capacity of moDCs to express genes for subunits of IL-12 family cytokines was monitored using real-time PCR and protein secretion in cell culture supernatants by ELISA. The percentage of CD83(+) moDCs was significantlyhigher in the allergic group after LPS stimulation (43.11 +/- 4.41) in comparison to the healthy group (24.85 +/- 3.37). Significantly higher gene expression of subunits of IL-12 family members was observed in moDCs of children of allerg

Název v anglickém jazyce

Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers

Popis výsledku anglicky

The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The maturation state (presence of CD83) of cord blood monocyte-derived dendritic cells (moDCs) 01 52 children of healthy mothers and 58 children of allergic mothers was estimated by flow cytometry. The capacity of moDCs to express genes for subunits of IL-12 family cytokines was monitored using real-time PCR and protein secretion in cell culture supernatants by ELISA. The percentage of CD83(+) moDCs was significantlyhigher in the allergic group after LPS stimulation (43.11 +/- 4.41) in comparison to the healthy group (24.85 +/- 3.37). Significantly higher gene expression of subunits of IL-12 family members was observed in moDCs of children of allerg

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/GD310%2F08%2FH077" target="_blank" >GD310/08/H077: Regulace imunologických mechanismů ve zdraví a nemoci: vývoj nových diagnostických a terapeutických postupů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5500

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

74-82

Kód UT WoS článku

000334986300004

EID výsledku v databázi Scopus

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