The effect of metronidazole on the cell cycle and DNA in metronidazole-susceptible and -resistant Giardia cell lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10295079" target="_blank" >RIV/00216208:11110/14:10295079 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0166685115000067" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0166685115000067</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molbiopara.2015.01.005" target="_blank" >10.1016/j.molbiopara.2015.01.005</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of metronidazole on the cell cycle and DNA in metronidazole-susceptible and -resistant Giardia cell lines
Popis výsledku v původním jazyce
Metronidazole (MTZ) is used as the drug of choice to treat Giardia infections. It is believed that the prodrug is transformed intracellularly into toxic intermediates that interact with cellular components, leading to cell death. The present study aimedto describe the effects of MTZ treatment on DNA and cell cycle progression in MTZ-sensitive and in vitro-derived MTZ-resistant cell lines. Detection of the phosphorylated form of histone H2A in cell nuclei together with electrophoresis of genomic DNA, flow cytometry analysis and incubation of cells with other drugs (albendazole or neomycin) demonstrated that DNA damage in MTZ-treated cells is clearly conditioned by the presence of this drug. The flow cytometry analysis and a BrdU labeling assay showed that the sublethal drug concentration affects the replication phase of the cell cycle. Cells incubated with lethal drug concentration exhibit unchanged DNA profile, only about 50% of cells are positive for gamma H2A and lose an ability to
Název v anglickém jazyce
The effect of metronidazole on the cell cycle and DNA in metronidazole-susceptible and -resistant Giardia cell lines
Popis výsledku anglicky
Metronidazole (MTZ) is used as the drug of choice to treat Giardia infections. It is believed that the prodrug is transformed intracellularly into toxic intermediates that interact with cellular components, leading to cell death. The present study aimedto describe the effects of MTZ treatment on DNA and cell cycle progression in MTZ-sensitive and in vitro-derived MTZ-resistant cell lines. Detection of the phosphorylated form of histone H2A in cell nuclei together with electrophoresis of genomic DNA, flow cytometry analysis and incubation of cells with other drugs (albendazole or neomycin) demonstrated that DNA damage in MTZ-treated cells is clearly conditioned by the presence of this drug. The flow cytometry analysis and a BrdU labeling assay showed that the sublethal drug concentration affects the replication phase of the cell cycle. Cells incubated with lethal drug concentration exhibit unchanged DNA profile, only about 50% of cells are positive for gamma H2A and lose an ability to
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Molecular and Biochemical Parasitology
ISSN
0166-6851
e-ISSN
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Svazek periodika
198
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
75-81
Kód UT WoS článku
000353096500004
EID výsledku v databázi Scopus
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