Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10282223" target="_blank" >RIV/00216208:11110/15:10282223 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00412-014-0486-5" target="_blank" >http://dx.doi.org/10.1007/s00412-014-0486-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00412-014-0486-5" target="_blank" >10.1007/s00412-014-0486-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Popis výsledku v původním jazyce

During mitotic prophase, chromosomes of the pathogenic unicellular eukaryote Giardia intestinalis condense in each of the cell's two nuclei. In this study, Giardia chromosomes were investigated using light microscopy, high-resolution field emission scanning electron microscopy, and in situ hybridization. For the first time, we describe the overall morphology, condensation stages, and mitotic segregation of these chromosomes. Despite the absence of several genes involved in the cohesion and condensationpathways in the Giardia genome, we observed chromatin organization similar to those found in eukaryotes, i.e., 10-nm nucleosomal fibrils, 30-nm fibrils coiled to chromomeres or in parallel arrangements, and closely aligned sister chromatids. DNA molecules of Giardia terminate with telomeric repeats that we visualized on each of the four chromatid endings of metaphase chromosomes. Giardia chromosomes lack primary and secondary constrictions, thus preventing their classification based on t

Název v anglickém jazyce

Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Popis výsledku anglicky

During mitotic prophase, chromosomes of the pathogenic unicellular eukaryote Giardia intestinalis condense in each of the cell's two nuclei. In this study, Giardia chromosomes were investigated using light microscopy, high-resolution field emission scanning electron microscopy, and in situ hybridization. For the first time, we describe the overall morphology, condensation stages, and mitotic segregation of these chromosomes. Despite the absence of several genes involved in the cohesion and condensationpathways in the Giardia genome, we observed chromatin organization similar to those found in eukaryotes, i.e., 10-nm nucleosomal fibrils, 30-nm fibrils coiled to chromomeres or in parallel arrangements, and closely aligned sister chromatids. DNA molecules of Giardia terminate with telomeric repeats that we visualized on each of the four chromatid endings of metaphase chromosomes. Giardia chromosomes lack primary and secondary constrictions, thus preventing their classification based on t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP305%2F12%2F1248" target="_blank" >GAP305/12/1248: Jak Giardia intestinalis, jednobuněčný eukaryot se dvěma jádry a minimalistickým genomem, zajišťuje integritu chromozómů</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromosoma

ISSN

0009-5915

e-ISSN

—

Svazek periodika

124

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

81-94

Kód UT WoS článku

000350304400007

EID výsledku v databázi Scopus

2-s2.0-84923699742