PCR diagnostic system in the treatment of prosthetic joint infections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10284820" target="_blank" >RIV/00216208:11110/15:10284820 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/15:10284820

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-014-0370-y" target="_blank" >http://dx.doi.org/10.1007/s12223-014-0370-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-014-0370-y" target="_blank" >10.1007/s12223-014-0370-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

PCR diagnostic system in the treatment of prosthetic joint infections

Popis výsledku v původním jazyce

In our prospective study, we examined whether a multiplex PCR diagnostic method is suitable for the primary detection of pathogens. We also examined the possibility and sensitivity of detecting genes responsible for biofilm production and methicillin resistance. From 2007 to 2009, 94 patients were included in the study. A UNB (universal detection of 16S ribosomal bacterial DNA) and UNF (universal detection of pathogenic fungi) were used in the primary detection. A multiplex assay for biofilm production,methicillin resistance allowed us to distinguish between Gram positivity and negativity and to detect Staphylococci. From all the samples, the culture was positive in 53.2 % of cases, and by using the UNB method, we detected bacteria in 79.8 % of cases-the UNF detection of fungi was positive in 10.6 % of cases. In 75 % of positive findings, we detected a Gram-negative bacterium in 65.3 % of cases. In 47.2 % of Staphylococci detected, the ability to produce biofilm was confirmed. 61.1 %

Název v anglickém jazyce

PCR diagnostic system in the treatment of prosthetic joint infections

Popis výsledku anglicky

In our prospective study, we examined whether a multiplex PCR diagnostic method is suitable for the primary detection of pathogens. We also examined the possibility and sensitivity of detecting genes responsible for biofilm production and methicillin resistance. From 2007 to 2009, 94 patients were included in the study. A UNB (universal detection of 16S ribosomal bacterial DNA) and UNF (universal detection of pathogenic fungi) were used in the primary detection. A multiplex assay for biofilm production,methicillin resistance allowed us to distinguish between Gram positivity and negativity and to detect Staphylococci. From all the samples, the culture was positive in 53.2 % of cases, and by using the UNB method, we detected bacteria in 79.8 % of cases-the UNF detection of fungi was positive in 10.6 % of cases. In 75 % of positive findings, we detected a Gram-negative bacterium in 65.3 % of cases. In 47.2 % of Staphylococci detected, the ability to produce biofilm was confirmed. 61.1 %

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FI - Traumatologie a ortopedie

OECD FORD obor

—

Projekt

<a href="/cs/project/NT14218" target="_blank" >NT14218: Využití antimikrobiálních peptidů k léčbě infekcí pohybového ústrojí za pomoci lokálních nosičů užívaných v ortopedii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

60

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

385-391

Kód UT WoS článku

000359398900003

EID výsledku v databázi Scopus

2-s2.0-84938997581