Analysis of Expression Profiles of Genes Involved in F0F1-ATP Synthase Biogenesis During Perinatal Development in Rat Liver and Skeletal Muscle
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10329192" target="_blank" >RIV/00216208:11110/16:10329192 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00064165:_____/16:10329192
Výsledek na webu
<a href="http://www.biomed.cas.cz/physiolres/pdf/65/65_597.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/65/65_597.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Analysis of Expression Profiles of Genes Involved in F0F1-ATP Synthase Biogenesis During Perinatal Development in Rat Liver and Skeletal Muscle
Popis výsledku v původním jazyce
During the process of intrauterine mammalian fetal development, the oxygen supply in growing fetus is low. A rapid switch from glycolysis based metabolism to oxidative phosphorylation (OXPHOS) must proceed during early postnatal adaptation to extra uterine conditions. Mitochondrial biogenesis and mammalian mitochondrial F0F1 ATP synthase assembly (complex V, EC 3.6.3.14, ATPase) are complex processes regulated by multiple transcription regulators and assembly factors. Using RNA expression analysis of rat liver and skeletal tissue (Rattus norvegicus, Berkenhout, 1769), we describe the expression profiles of 20 genes involved in mitochondrial maturation and ATP synthase biogenesis in detail between the 16th and 22nd day of gestation and the first 4 days of life. We observed that the most important expression shift occurred in the liver between the 20th and 22nd day of gestation, indicating that the fetus prepares for birth about two days before parturition. The detailed mechanism regulating the perinatal adaptation process is not yet known. Deeper insights in perinatal physiological development will help to assess mitochondrial dysfunction in the broader context of cell metabolism in preterm newborns or neonates with poor adaptation to extra uterine life.
Název v anglickém jazyce
Analysis of Expression Profiles of Genes Involved in F0F1-ATP Synthase Biogenesis During Perinatal Development in Rat Liver and Skeletal Muscle
Popis výsledku anglicky
During the process of intrauterine mammalian fetal development, the oxygen supply in growing fetus is low. A rapid switch from glycolysis based metabolism to oxidative phosphorylation (OXPHOS) must proceed during early postnatal adaptation to extra uterine conditions. Mitochondrial biogenesis and mammalian mitochondrial F0F1 ATP synthase assembly (complex V, EC 3.6.3.14, ATPase) are complex processes regulated by multiple transcription regulators and assembly factors. Using RNA expression analysis of rat liver and skeletal tissue (Rattus norvegicus, Berkenhout, 1769), we describe the expression profiles of 20 genes involved in mitochondrial maturation and ATP synthase biogenesis in detail between the 16th and 22nd day of gestation and the first 4 days of life. We observed that the most important expression shift occurred in the liver between the 20th and 22nd day of gestation, indicating that the fetus prepares for birth about two days before parturition. The detailed mechanism regulating the perinatal adaptation process is not yet known. Deeper insights in perinatal physiological development will help to assess mitochondrial dysfunction in the broader context of cell metabolism in preterm newborns or neonates with poor adaptation to extra uterine life.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GB14-36804G" target="_blank" >GB14-36804G: Centrum mitochondriální biologie a patologie (MITOCENTRUM)</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Physiological Research
ISSN
0862-8408
e-ISSN
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Svazek periodika
65
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
12
Strana od-do
597-608
Kód UT WoS článku
000386685600007
EID výsledku v databázi Scopus
2-s2.0-84997501404