Quantification of ethosuximide in human serum by GC-MS after butyl chloroformate derivatization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10336907" target="_blank" >RIV/00216208:11110/16:10336907 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10336907

Výsledek na webu

<a href="http://web.natur.cuni.cz/analchem/isc/isc12.pdf" target="_blank" >http://web.natur.cuni.cz/analchem/isc/isc12.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of ethosuximide in human serum by GC-MS after butyl chloroformate derivatization

Popis výsledku v původním jazyce

A simple, sensitive and robust method for determination of anti-epileptic drug ethosuximide in human serum using GC-MS was developed and validated for clinical toxicology purposes. This method employs an emerging class of derivatization agents: alkyl chloro-formates allowing the efficient and rapid derivatization of both the amino and carboxylic groups of the tested antiepileptic drug within seconds. The derivatization protocol was optimized using the Design of Experiment, and the entire sample preparation requires less than 5 minutes. Linear calibration curves were obtained in the concentration range 0.5-150 mu g/mL, with adequate accuracy (98.0-109.0%) and precision (< 10.4%). The method was successfully applied to quantification of ethosuximide in the serum of patients in both therapeutic and toxic concentration ranges

Název v anglickém jazyce

Quantification of ethosuximide in human serum by GC-MS after butyl chloroformate derivatization

Popis výsledku anglicky

A simple, sensitive and robust method for determination of anti-epileptic drug ethosuximide in human serum using GC-MS was developed and validated for clinical toxicology purposes. This method employs an emerging class of derivatization agents: alkyl chloro-formates allowing the efficient and rapid derivatization of both the amino and carboxylic groups of the tested antiepileptic drug within seconds. The derivatization protocol was optimized using the Design of Experiment, and the entire sample preparation requires less than 5 minutes. Linear calibration curves were obtained in the concentration range 0.5-150 mu g/mL, with adequate accuracy (98.0-109.0%) and precision (< 10.4%). The method was successfully applied to quantification of ethosuximide in the serum of patients in both therapeutic and toxic concentration ranges

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROCEEDINGS OF THE 12TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY

ISBN

978-80-7444-044-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

17-22

Název nakladatele

CHARLES UNIV, FAC SCIENCE

Místo vydání

PRAGUE 2

Místo konání akce

Prague

Datum konání akce

22. 9. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000391253800004