Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361459" target="_blank" >RIV/00216208:11110/17:10361459 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/17:43913179 RIV/00064173:_____/17:N0000172 RIV/00023752:_____/17:43919218 RIV/00064165:_____/17:10361459

Výsledek na webu

<a href="http://dx.doi.org/10.5507/bp.2017.017" target="_blank" >http://dx.doi.org/10.5507/bp.2017.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2017.017" target="_blank" >10.5507/bp.2017.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients

Popis výsledku v původním jazyce

Aims. To optimise the ELISA method for the avidity of IgG antibodies against neurofilament heavy chain (NfH) and to determine the levels and avidity of anti-NfH antibodies in patients with Alzheimer&apos;s disease (AD) and a healthy control group. Methods. Various dilutions of sera and concentrations of urea and sodium chloride as chaotropic reagents were tested in the process of the ELISA optimisation. The levels and avidity of anti-NfH antibodies were determined in 30 patients with Alzheimer&apos;s disease and 30 age-matched cognitively normal elderly adults. Results. Sera dilution 1: 200 and urea as a chaotrope in a concentration 6 mol/L were chosen to be the most suitable for the avidity assay of anti-NfH antibodies by ELISA. The results showed no differences in either level or avidity of IgG anti-NfH antibodies between AD patients and cognitively normal persons. The levels of anti-NfH IgG antibodies inversely correlated with their avidities.Conclusions. We optimised the ELISA method for the determination of anti-NfH antibody avidity determination which is suitable for research of anti-NfH antibody avidity in patients with neurological diseases associated with neurocytoskeletal defects. The determination of serum anti-NfH antibody avidity in AD patients seems to have limited diagnostic significance.

Název v anglickém jazyce

Avidity of antineurocytoskeletal antibodies in Alzheimer's disease patients

Popis výsledku anglicky

Aims. To optimise the ELISA method for the avidity of IgG antibodies against neurofilament heavy chain (NfH) and to determine the levels and avidity of anti-NfH antibodies in patients with Alzheimer&apos;s disease (AD) and a healthy control group. Methods. Various dilutions of sera and concentrations of urea and sodium chloride as chaotropic reagents were tested in the process of the ELISA optimisation. The levels and avidity of anti-NfH antibodies were determined in 30 patients with Alzheimer&apos;s disease and 30 age-matched cognitively normal elderly adults. Results. Sera dilution 1: 200 and urea as a chaotrope in a concentration 6 mol/L were chosen to be the most suitable for the avidity assay of anti-NfH antibodies by ELISA. The results showed no differences in either level or avidity of IgG anti-NfH antibodies between AD patients and cognitively normal persons. The levels of anti-NfH IgG antibodies inversely correlated with their avidities.Conclusions. We optimised the ELISA method for the determination of anti-NfH antibody avidity determination which is suitable for research of anti-NfH antibody avidity in patients with neurological diseases associated with neurocytoskeletal defects. The determination of serum anti-NfH antibody avidity in AD patients seems to have limited diagnostic significance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

—

Svazek periodika

161

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

179-186

Kód UT WoS článku

000406522700009

EID výsledku v databázi Scopus

2-s2.0-85020548974