Brain region-specific effects of immobilization stress on cholinesterases in mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10361961" target="_blank" >RIV/00216208:11110/17:10361961 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10253890.2016.1263836" target="_blank" >http://dx.doi.org/10.1080/10253890.2016.1263836</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10253890.2016.1263836" target="_blank" >10.1080/10253890.2016.1263836</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Brain region-specific effects of immobilization stress on cholinesterases in mice

Popis výsledku v původním jazyce

Brain acetylcholinesterase (AChE) variant AChE(R) expression increases with acute stress, and this persists for an extended period, although the timing, strain and laterality differences, have not been explored previously. Acute stress transiently increases acetylcholine release, which in turn may increase activity of cholinesterases. Also the AChE gene contains a glucocorticoid response element (GRE), and stress-inducible AChE transcription and activity changes are linked to increased glucocorticoid levels. Corticotropin-releasing hormone knockout (CRH-KO) mice have basal glucocorticoid levels similar to wild type (WT) mice, but much lower levels during stress. Hence we hypothesized that CRH is important for the cholinesterase stress responses, including butyrylcholinesterase (BChE). We used immobilization stress, acute (30 or 120min) and repeated (120min dailyx7) in 48 male mice (24WT and 24 CRH-KO) and determined AChE(R), AChE and BChE mRNA expression and AChE and BChE activities in left and right brain areas (as cholinergic signaling shows laterality). Immobilization decreased BChE mRNA expression (right amygdala, to 0.5, 0.3 and 0.4, xcontrol respectively) and AChE(R) mRNA expression (to 0.5, 0.4 and 0.4, xcontrol respectively). AChE mRNA expression increased (1.3, 1.4 and 1.8-fold, respectively) in the left striatum (Str). The AChE activity increased in left Str (after 30min, 1.2-fold), decreased in right parietal cortex with repeated stress (to 0.5xcontrol). BChE activity decreased after 30min in the right CA3 region (to 0.4xcontrol) but increased (3.8-fold) after 120min in the left CA3 region. The pattern of changes in CRH-KO differed from that in WT mice.

Název v anglickém jazyce

Brain region-specific effects of immobilization stress on cholinesterases in mice

Popis výsledku anglicky

Brain acetylcholinesterase (AChE) variant AChE(R) expression increases with acute stress, and this persists for an extended period, although the timing, strain and laterality differences, have not been explored previously. Acute stress transiently increases acetylcholine release, which in turn may increase activity of cholinesterases. Also the AChE gene contains a glucocorticoid response element (GRE), and stress-inducible AChE transcription and activity changes are linked to increased glucocorticoid levels. Corticotropin-releasing hormone knockout (CRH-KO) mice have basal glucocorticoid levels similar to wild type (WT) mice, but much lower levels during stress. Hence we hypothesized that CRH is important for the cholinesterase stress responses, including butyrylcholinesterase (BChE). We used immobilization stress, acute (30 or 120min) and repeated (120min dailyx7) in 48 male mice (24WT and 24 CRH-KO) and determined AChE(R), AChE and BChE mRNA expression and AChE and BChE activities in left and right brain areas (as cholinergic signaling shows laterality). Immobilization decreased BChE mRNA expression (right amygdala, to 0.5, 0.3 and 0.4, xcontrol respectively) and AChE(R) mRNA expression (to 0.5, 0.4 and 0.4, xcontrol respectively). AChE mRNA expression increased (1.3, 1.4 and 1.8-fold, respectively) in the left striatum (Str). The AChE activity increased in left Str (after 30min, 1.2-fold), decreased in right parietal cortex with repeated stress (to 0.5xcontrol). BChE activity decreased after 30min in the right CA3 region (to 0.4xcontrol) but increased (3.8-fold) after 120min in the left CA3 region. The pattern of changes in CRH-KO differed from that in WT mice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Stress: the International Journal on Biology of Stress

ISSN

1025-3890

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

36-43

Kód UT WoS článku

000395242300006

EID výsledku v databázi Scopus

2-s2.0-85002291952