Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10363163" target="_blank" >RIV/00216208:11110/17:10363163 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/17:10363163 RIV/00064165:_____/17:10363163

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41598-017-02905-8" target="_blank" >http://dx.doi.org/10.1038/s41598-017-02905-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-02905-8" target="_blank" >10.1038/s41598-017-02905-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Popis výsledku v původním jazyce

Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-alpha m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-alpha mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 +/- 1.1 vs. 0.86 +/- 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-alpha mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-a mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.

Název v anglickém jazyce

Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Popis výsledku anglicky

Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-alpha m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-alpha mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 +/- 1.1 vs. 0.86 +/- 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-alpha mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-a mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

June

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000402508300002

EID výsledku v databázi Scopus

2-s2.0-85020163461