Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10364251" target="_blank" >RIV/00216208:11110/17:10364251 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023728:_____/17:N0000049

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cyto.2017.06.009" target="_blank" >http://dx.doi.org/10.1016/j.cyto.2017.06.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cyto.2017.06.009" target="_blank" >10.1016/j.cyto.2017.06.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis

Popis výsledku v původním jazyce

Background: Interleukin (IL)-20 is a pro-inflammatory cytokine that may be implicated in the pathogenesis of rheumatoid arthritis (RA). This study aimed to determine the association between IL-20 and disease activity in patients with RA. Methods: The levels of serum and synovial fluid IL-20 were measured in patients with RA and OA. The disease activity was assessed based on the Disease Activity Score of 28 joints (DAS28). The expression of IL-20 in synovial tissue samples from patients with RA and OA were determined by immunohistochemistry. Immunofluorescence staining was used to co-localize IL-20 with selected cells. The secretion of IL-20 was analysed in human peripheral blood mononuclear cells (PBMCs) of patients with RA. Results: Synovial fluid and synovial tissue IL-20 were significantly increased in patients with RA compared with patients with OA. The expression of IL-20 in RA synovial tissue was particularly associated with macrophages and neutrophil granulocytes, but also with synovial fibroblasts and lymphocytes. The IL-20 levels in synovial fluid correlated with DAS28 (r = 0.434; p = 0.015) and were significantly elevated in anti-CCP positive RA compared with anti-CCP negative RA (122.3 +/- 104.1 pg/ml and 45.9 +/- 35.8 pg/ml; p = 0.008). IL-20 production from PBMCs was induced by Poly I:C and LPS but not with pro-inflammatory cytokines, such as TNF-alpha or IL-1. Conclusion: Our data showed that IL-20 is independently associated with RA disease activity and may be triggered by TLR ligands at local sites of inflammation.

Název v anglickém jazyce

Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis

Popis výsledku anglicky

Background: Interleukin (IL)-20 is a pro-inflammatory cytokine that may be implicated in the pathogenesis of rheumatoid arthritis (RA). This study aimed to determine the association between IL-20 and disease activity in patients with RA. Methods: The levels of serum and synovial fluid IL-20 were measured in patients with RA and OA. The disease activity was assessed based on the Disease Activity Score of 28 joints (DAS28). The expression of IL-20 in synovial tissue samples from patients with RA and OA were determined by immunohistochemistry. Immunofluorescence staining was used to co-localize IL-20 with selected cells. The secretion of IL-20 was analysed in human peripheral blood mononuclear cells (PBMCs) of patients with RA. Results: Synovial fluid and synovial tissue IL-20 were significantly increased in patients with RA compared with patients with OA. The expression of IL-20 in RA synovial tissue was particularly associated with macrophages and neutrophil granulocytes, but also with synovial fibroblasts and lymphocytes. The IL-20 levels in synovial fluid correlated with DAS28 (r = 0.434; p = 0.015) and were significantly elevated in anti-CCP positive RA compared with anti-CCP negative RA (122.3 +/- 104.1 pg/ml and 45.9 +/- 35.8 pg/ml; p = 0.008). IL-20 production from PBMCs was induced by Poly I:C and LPS but not with pro-inflammatory cytokines, such as TNF-alpha or IL-1. Conclusion: Our data showed that IL-20 is independently associated with RA disease activity and may be triggered by TLR ligands at local sites of inflammation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30226 - Rheumatology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytokine

ISSN

1043-4666

e-ISSN

—

Svazek periodika

97

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

187-192

Kód UT WoS článku

000406726600023

EID výsledku v databázi Scopus

2-s2.0-85021107873