Neural crest contribution to the avian shoulder girdle and implications to girdle evolution in vertebrates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10366797" target="_blank" >RIV/00216208:11110/17:10366797 - isvavai.cz</a>

Výsledek na webu

<a href="https://biocomm.spbu.ru/article/view/6/11" target="_blank" >https://biocomm.spbu.ru/article/view/6/11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21638/11701/spbu03.2017.104" target="_blank" >10.21638/11701/spbu03.2017.104</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Neural crest contribution to the avian shoulder girdle and implications to girdle evolution in vertebrates

Popis výsledku v původním jazyce

Neural crest (NC) is an established source for many endochondral and intramembranous bones in the skull and postcranial skeleton in vertebrates. Neural crest cells also contribute to the trapezius/cleidohyoideus muscle attachment sites on the shoulder girdle of the mouse, where they are found in the scapula, clavicle, and sternum. In the avian shoulder girdle, NC cells from the level of the first two cranial-most somites were only found so far in the clavicle, while in the axolotl, the NC contribution to the shoulder girdle was not found at all. In this study we aimed to determine whether NC cells caudal to the level of the second somite contribute to the cartilaginous shoulder girdle in birds and to analyse the phylogenetic distribution of NC cells in the vertebrate shoulder girdle. Homotopic quail to chick embryos and GFP+ to white chick embryos transplantations of the neural tube including presumptive NC, as well as immunohistochemical detection of NC markers, such as HNK-1 and PDGFRα revealed no contribution of NC cells from somite levels 3-27 to the skeletal elements of the shoulder girdle, including, but not restricting to muscle attachment sites, despite abundance of other NC derivatives. Thus, in birds, NC does not contribute to the formation of the cartilaginous shoulder girdle. The negative result is discussed in a broad evolutionary aspect. It supports the notion of the uniqueness of NC contribution to the variety of endochondral bones in mice (or mammals). In other vertebrates, including birds, only the cells of the cranial NC seem to migrate to the shoulder girdle and contribute to the intramembranous clavicles and/or interclavicle. We critically evaluate the existing hypotheses on evolution of NC contribution to the shoulder girdle in vertebrates.

Název v anglickém jazyce

Neural crest contribution to the avian shoulder girdle and implications to girdle evolution in vertebrates

Popis výsledku anglicky

Neural crest (NC) is an established source for many endochondral and intramembranous bones in the skull and postcranial skeleton in vertebrates. Neural crest cells also contribute to the trapezius/cleidohyoideus muscle attachment sites on the shoulder girdle of the mouse, where they are found in the scapula, clavicle, and sternum. In the avian shoulder girdle, NC cells from the level of the first two cranial-most somites were only found so far in the clavicle, while in the axolotl, the NC contribution to the shoulder girdle was not found at all. In this study we aimed to determine whether NC cells caudal to the level of the second somite contribute to the cartilaginous shoulder girdle in birds and to analyse the phylogenetic distribution of NC cells in the vertebrate shoulder girdle. Homotopic quail to chick embryos and GFP+ to white chick embryos transplantations of the neural tube including presumptive NC, as well as immunohistochemical detection of NC markers, such as HNK-1 and PDGFRα revealed no contribution of NC cells from somite levels 3-27 to the skeletal elements of the shoulder girdle, including, but not restricting to muscle attachment sites, despite abundance of other NC derivatives. Thus, in birds, NC does not contribute to the formation of the cartilaginous shoulder girdle. The negative result is discussed in a broad evolutionary aspect. It supports the notion of the uniqueness of NC contribution to the variety of endochondral bones in mice (or mammals). In other vertebrates, including birds, only the cells of the cranial NC seem to migrate to the shoulder girdle and contribute to the intramembranous clavicles and/or interclavicle. We critically evaluate the existing hypotheses on evolution of NC contribution to the shoulder girdle in vertebrates.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biological Communications

ISSN

2542-2154

e-ISSN

—

Svazek periodika

62

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RU - Ruská federace

Počet stran výsledku

12

Strana od-do

26-37

Kód UT WoS článku

—

EID výsledku v databázi Scopus

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