Novel Approaches to Study Coronary Vasculature Development in Mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378062" target="_blank" >RIV/00216208:11110/18:10378062 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/18:00493023

Výsledek na webu

<a href="https://doi.org/10.1002/DVDY.24637" target="_blank" >https://doi.org/10.1002/DVDY.24637</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/DVDY.24637" target="_blank" >10.1002/DVDY.24637</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel Approaches to Study Coronary Vasculature Development in Mice

Popis výsledku v původním jazyce

Background: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. Results: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. Conclusions: The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models.

Název v anglickém jazyce

Novel Approaches to Study Coronary Vasculature Development in Mice

Popis výsledku anglicky

Background: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. Results: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. Conclusions: The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/GA13-12412S" target="_blank" >GA13-12412S: Vývoj převodního systému u myši analyzovaný 3D zobrazovacími metodami a optickým mapováním</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Dynamics

ISSN

1058-8388

e-ISSN

—

Svazek periodika

247

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1018-1027

Kód UT WoS článku

000441275600005

EID výsledku v databázi Scopus

2-s2.0-85050401942