The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10381664" target="_blank" >RIV/00216208:11110/18:10381664 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1017/S0031182018000409" target="_blank" >https://doi.org/10.1017/S0031182018000409</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182018000409" target="_blank" >10.1017/S0031182018000409</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic

Popis výsledku v původním jazyce

To date, genotyping data on giardiasis has not been available in the Czech Republic. In this study, we characterised 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase, and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and 6 (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterised by larger genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (mainly drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant correlations with the clinical data were found.

Název v anglickém jazyce

The first multilocus genotype analysis of Giardia intestinalis in humans in the Czech Republic

Popis výsledku anglicky

To date, genotyping data on giardiasis has not been available in the Czech Republic. In this study, we characterised 47 human isolates of Giardia intestinalis from symptomatic as well as asymptomatic giardiasis cases. Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (β-giardin, glutamate dehydrogenase, and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and 6 (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterised by larger genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (mainly drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant correlations with the clinical data were found.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/NV15-33369A" target="_blank" >NV15-33369A: Využití vlastností izolátů Giardia intestinalis, původce nejběžnější střevní protozoární nákazy, pro diagnostiku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

—

Svazek periodika

145

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1577-1587

Kód UT WoS článku

000445854900011

EID výsledku v databázi Scopus

2-s2.0-85044213267