The deletion of M-4 muscarinic receptors increases motor activity in females in the dark phase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10382866" target="_blank" >RIV/00216208:11110/18:10382866 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/18:00496886

Výsledek na webu

<a href="https://doi.org/10.1002/brb3.1057" target="_blank" >https://doi.org/10.1002/brb3.1057</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/brb3.1057" target="_blank" >10.1002/brb3.1057</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The deletion of M-4 muscarinic receptors increases motor activity in females in the dark phase

Popis výsledku v původním jazyce

Objectives: M-4 muscarinic receptors (MR) presumably play a role in motor coordination. Previous studies have shown different results depending on genetic background and number of backcrosses. However, no attention has been given to biorhythms. Material and Methods: We therefore analyzed biorhythms under a light/dark cycle obtained telemetrically in intact animals (activity, body temperature) in M4KO mice growth on the C57Bl6 background using ChronosFit software. Studying pure effects of gene knockout in daily rhythms is especially important knowledge for pharmacological/behavioral studies in which drugs are usually tested in the morning. Results: We show that M4KO mice motor activity does not differ substantially from wild-type mice during light period while in the dark phase (mice active part of the day), the M4KO mice reveal biorhythm changes in many parameters. Moreover, these differences are sex-dependent and are evident in females only. Mesor, night-day difference, and night value were doubled or tripled when comparing female KO versus male KO. Our in vitro autoradiography demonstrates that M4MR proportion represents 24% in the motor cortex (MOCx), 30% in the somatosensory cortex, 50% in the striatum, 69% in the thalamus, and 48% in the intergeniculate leaflet (IGL). The M4MR densities were negligible in the subparaventricular zone, the posterior hypothalamic area, and in the suprachiasmatic nuclei. Conclusions: We conclude that cholinergic signaling at M4MR in brain structures such as striatum, MOCx, and probably with the important participation of IGL significantly control motor activity biorhythm. Animal activity differs in the light and dark phases, which should be taken into consideration when interpreting the results.

Název v anglickém jazyce

The deletion of M-4 muscarinic receptors increases motor activity in females in the dark phase

Popis výsledku anglicky

Objectives: M-4 muscarinic receptors (MR) presumably play a role in motor coordination. Previous studies have shown different results depending on genetic background and number of backcrosses. However, no attention has been given to biorhythms. Material and Methods: We therefore analyzed biorhythms under a light/dark cycle obtained telemetrically in intact animals (activity, body temperature) in M4KO mice growth on the C57Bl6 background using ChronosFit software. Studying pure effects of gene knockout in daily rhythms is especially important knowledge for pharmacological/behavioral studies in which drugs are usually tested in the morning. Results: We show that M4KO mice motor activity does not differ substantially from wild-type mice during light period while in the dark phase (mice active part of the day), the M4KO mice reveal biorhythm changes in many parameters. Moreover, these differences are sex-dependent and are evident in females only. Mesor, night-day difference, and night value were doubled or tripled when comparing female KO versus male KO. Our in vitro autoradiography demonstrates that M4MR proportion represents 24% in the motor cortex (MOCx), 30% in the somatosensory cortex, 50% in the striatum, 69% in the thalamus, and 48% in the intergeniculate leaflet (IGL). The M4MR densities were negligible in the subparaventricular zone, the posterior hypothalamic area, and in the suprachiasmatic nuclei. Conclusions: We conclude that cholinergic signaling at M4MR in brain structures such as striatum, MOCx, and probably with the important participation of IGL significantly control motor activity biorhythm. Animal activity differs in the light and dark phases, which should be taken into consideration when interpreting the results.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/GA17-03847S" target="_blank" >GA17-03847S: Role M4 muskarinových receptorů v regulaci biorytmů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Brain and Behavior

ISSN

2162-3279

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000449070600019

EID výsledku v databázi Scopus

2-s2.0-85050912034