Distinct characteristics of Tregs of newborns of healthy and allergic mothers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10385531" target="_blank" >RIV/00216208:11110/18:10385531 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023698:_____/18:N0000036

Výsledek na webu

<a href="https://doi.org/10.1371/journal.pone.0207998" target="_blank" >https://doi.org/10.1371/journal.pone.0207998</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0207998" target="_blank" >10.1371/journal.pone.0207998</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Distinct characteristics of Tregs of newborns of healthy and allergic mothers

Popis výsledku v původním jazyce

Allergic diseases represent a major issue in clinical and experimental immunology due to their high and increasing incidence worldwide. Allergy status of the mother remains the best predictor of an individual&apos;s increased risk of allergy development. Dysregulation of the balance between different branches of immune response, chiefly excessive polarization towards Th2, is the underlying cause of allergic diseases. Regulatory T cells (Tregs) play a pivotal role in the timely establishment of physiological immune polarization and are crucial for control of allergy. In our study we used flow cytometry to assess Tregs in cord blood of newborns of healthy (n = 121) and allergic (n = 108) mothers. We observed a higher percentage of Tregs (CD4(+) CD25(+) CD127(low) FoxP3(+)) in cord blood of children of allergic mothers. However, the percentage of cells expressing extracellular (PD-1, CTLA-4, GITR) and intracellular (IL-10, TGF-beta) markers of function was lower (significantly for PD-1 and IL-10) within Tregs of these children. Furthermore, Helios - induced Tregs in the cord blood of children of allergic mothers were decreased. These results were supported by a decrease in plasma levels of IL-10 and TGF-beta in cord blood of newborns of allergic mothers, implying lower tolerogenic capacity on the systemic level. Taken together, these findings reflect deficient function of Tregs in the group with higher risk of allergy development. This may be caused by a lower maturation status of the immune system, specifically Tregs, at birth. Such immaturity may represent an important mechanism involved in the increased risk of allergy in children of allergic mothers.

Název v anglickém jazyce

Distinct characteristics of Tregs of newborns of healthy and allergic mothers

Popis výsledku anglicky

Allergic diseases represent a major issue in clinical and experimental immunology due to their high and increasing incidence worldwide. Allergy status of the mother remains the best predictor of an individual&apos;s increased risk of allergy development. Dysregulation of the balance between different branches of immune response, chiefly excessive polarization towards Th2, is the underlying cause of allergic diseases. Regulatory T cells (Tregs) play a pivotal role in the timely establishment of physiological immune polarization and are crucial for control of allergy. In our study we used flow cytometry to assess Tregs in cord blood of newborns of healthy (n = 121) and allergic (n = 108) mothers. We observed a higher percentage of Tregs (CD4(+) CD25(+) CD127(low) FoxP3(+)) in cord blood of children of allergic mothers. However, the percentage of cells expressing extracellular (PD-1, CTLA-4, GITR) and intracellular (IL-10, TGF-beta) markers of function was lower (significantly for PD-1 and IL-10) within Tregs of these children. Furthermore, Helios - induced Tregs in the cord blood of children of allergic mothers were decreased. These results were supported by a decrease in plasma levels of IL-10 and TGF-beta in cord blood of newborns of allergic mothers, implying lower tolerogenic capacity on the systemic level. Taken together, these findings reflect deficient function of Tregs in the group with higher risk of allergy development. This may be caused by a lower maturation status of the immune system, specifically Tregs, at birth. Such immaturity may represent an important mechanism involved in the increased risk of allergy in children of allergic mothers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30225 - Allergy

Projekt

<a href="/cs/project/NV15-26877A" target="_blank" >NV15-26877A: Vliv časného postnatálního osídlení novorozenců E. coli na výskyt alergie, vybraných imunologických charakteristik a složení střevní mikrobioty</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

—

Kód UT WoS článku

000451325700099

EID výsledku v databázi Scopus

2-s2.0-85057155808