Direct tryptic cleavage in bone tissue followed by LC-MS/MS as a first step towards routine characterization of proteins embedded in alveolar bones

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10412848" target="_blank" >RIV/00216208:11110/20:10412848 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/20:43921484 RIV/60461373:22340/20:43921484

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0V-rAjjyTz" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0V-rAjjyTz</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijms.2020.116375" target="_blank" >10.1016/j.ijms.2020.116375</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct tryptic cleavage in bone tissue followed by LC-MS/MS as a first step towards routine characterization of proteins embedded in alveolar bones

Popis výsledku v původním jazyce

While methods like radiology or histology are routinely used in oral surgery, a simple and effective method for characterization of alveolar bones at the molecular level is missing. This is primarily due to the fact that proteomic analysis of hard tissue represents a serious analytical challenge. In this study, we developed a simple technique for the proteomic characterization of bone tissue in the field of oral surgery. After a prewashing step with different solvents, specific digestion with trypsin was performed directly in porcine alveolar bone tissues without the prior reduction and alkylation of disulfide bonds. The released peptides were then analyzed using liquid chromatography-tandem mass spectrometry. When 2-propanol was used in the pre-incubation step, this approach identified 487 proteins in the maxillary (upper jaw) bone and 443 proteins in the mandibular (lower jaw) bone, 40 of each being specifically ascribed to bone tissue. This suggests that routine use of our technique can provide deeper insights into the field of oral surgery at the molecular level. Furthermore, it could also have diagnostic potential in differentiating between the proteomic patterns of healthy and pathological alveolar bone tissue. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD018836.

Název v anglickém jazyce

Direct tryptic cleavage in bone tissue followed by LC-MS/MS as a first step towards routine characterization of proteins embedded in alveolar bones

Popis výsledku anglicky

While methods like radiology or histology are routinely used in oral surgery, a simple and effective method for characterization of alveolar bones at the molecular level is missing. This is primarily due to the fact that proteomic analysis of hard tissue represents a serious analytical challenge. In this study, we developed a simple technique for the proteomic characterization of bone tissue in the field of oral surgery. After a prewashing step with different solvents, specific digestion with trypsin was performed directly in porcine alveolar bone tissues without the prior reduction and alkylation of disulfide bonds. The released peptides were then analyzed using liquid chromatography-tandem mass spectrometry. When 2-propanol was used in the pre-incubation step, this approach identified 487 proteins in the maxillary (upper jaw) bone and 443 proteins in the mandibular (lower jaw) bone, 40 of each being specifically ascribed to bone tissue. This suggests that routine use of our technique can provide deeper insights into the field of oral surgery at the molecular level. Furthermore, it could also have diagnostic potential in differentiating between the proteomic patterns of healthy and pathological alveolar bone tissue. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD018836.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LTAIN19007" target="_blank" >LTAIN19007: Vývoj pokročilých výpočetních algoritmů pro objektivní posouzení pooperační rehabilitace</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Mass Spectrometry

ISSN

1387-3806

e-ISSN

—

Svazek periodika

455

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

116375

Kód UT WoS článku

000555567000008

EID výsledku v databázi Scopus

—