A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10471891" target="_blank" >RIV/00216208:11110/24:10471891 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/23:43928218 RIV/00064165:_____/24:10471891 RIV/60461373:22340/24:43928218

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CeikzEViaL" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CeikzEViaL</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/open.202300147" target="_blank" >10.1002/open.202300147</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Popis výsledku v původním jazyce

A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 mu g/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro. A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media. Separation was performed using a gradient on a C18 column with a mobile phase of acetonitrile and acidified miliQ water. The method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, as well as for robustness. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.image

Název v anglickém jazyce

A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media

Popis výsledku anglicky

A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 mu g/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro. A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media. Separation was performed using a gradient on a C18 column with a mobile phase of acetonitrile and acidified miliQ water. The method was validated for linearity, accuracy, precision, limit of quantitation, limit of detection, as well as for robustness. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.image

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemistryOpen

ISSN

2191-1363

e-ISSN

2191-1363

Svazek periodika

13

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

e202300147

Kód UT WoS článku

001100702900001

EID výsledku v databázi Scopus

2-s2.0-85176381229