Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10486422" target="_blank" >RIV/00216208:11110/24:10486422 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/24:10486422 RIV/00064165:_____/24:10486422

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=l9eIlxK48K" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=l9eIlxK48K</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13059-024-03396-3" target="_blank" >10.1186/s13059-024-03396-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

Popis výsledku v původním jazyce

Background: Embryos are regeneration and wound healing masters. They rapidly close wounds and scarlessly remodel and regenerate injured tissue. Regeneration has been extensively studied in many animal models using new tools such as single-cell analysis. However, until now, they have been based primarily on experiments assessing from 1 day post injury. Results: In this paper, we reveal that critical steps initiating regeneration occur within hours after injury. We discovered the regeneration initiating cells (RICs) using single-cell and spatial transcriptomics of the regenerating Xenopus laevis tail. RICs are formed transiently from the basal epidermal cells, and their expression signature suggests they are important for modifying the surrounding extracellular matrix thus regulating development. The absence or deregulation of RICs leads to excessive extracellular matrix deposition and defective regeneration. Conclusion: RICs represent a newly discovered transient cell state involved in the initiation of the regeneration process.

Název v anglickém jazyce

Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

Popis výsledku anglicky

Background: Embryos are regeneration and wound healing masters. They rapidly close wounds and scarlessly remodel and regenerate injured tissue. Regeneration has been extensively studied in many animal models using new tools such as single-cell analysis. However, until now, they have been based primarily on experiments assessing from 1 day post injury. Results: In this paper, we reveal that critical steps initiating regeneration occur within hours after injury. We discovered the regeneration initiating cells (RICs) using single-cell and spatial transcriptomics of the regenerating Xenopus laevis tail. RICs are formed transiently from the basal epidermal cells, and their expression signature suggests they are important for modifying the surrounding extracellular matrix thus regulating development. The absence or deregulation of RICs leads to excessive extracellular matrix deposition and defective regeneration. Conclusion: RICs represent a newly discovered transient cell state involved in the initiation of the regeneration process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome Biology

ISSN

1474-7596

e-ISSN

1474-760X

Svazek periodika

25

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

28

Strana od-do

251

Kód UT WoS článku

001325705900001

EID výsledku v databázi Scopus

2-s2.0-85205446195