Effect of TiO2 Nanoparticles Defect Structure on their ROS Scavenging Ability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10496511" target="_blank" >RIV/00216208:11110/24:10496511 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1109/NAP62956.2024.10739734" target="_blank" >https://doi.org/10.1109/NAP62956.2024.10739734</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/NAP62956.2024.10739734" target="_blank" >10.1109/NAP62956.2024.10739734</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of TiO2 Nanoparticles Defect Structure on their ROS Scavenging Ability

Popis výsledku v původním jazyce

Titanium dioxide nanoparticles (TiO2 NPs) have attracted significant interest for their ability to modulate reactive oxygen species (ROS) levels within cells. These nanoparticles have shown promise in drug delivery, sonodynamic, photodynamic, photothermal, and ionizing radiation-based anti-cancer therapies, as well as antimicrobial and antioxidant applications. In this study, we analyzed the effects of TiO2 NP defect structures-specifically the presence of stoichiometric (Ti4+) and non-stoichiometric (Ti-3. and Ti-2.) titanium ions within the crystal lattice-and the aggregation state of TiO2 NPs on their ROS scavenging capabilities in both cell-free conditions and H2O2-treated L929 cells. Two types of TiO2 NPs with varying concentrations of Ti-3. and Ti-2. ions were synthesized and characterized using XRD, TEM, SAXS, and XPS techniques. The antioxidant properties of these TiO2 NPs were assessed through chemiluminescence and optical spectroscopy using ROS sensors. Results from chemiluminescence and total antioxidant capacity assays indicated that the synthesized TiO2 NPs possess radical scavenging abilities, with TiO2 NPs(1), containing a higher amount of non- stoichiometric titanium ions, demonstrating a stronger effect. Concentrations of up to 40 mg/L of TiO2 NPs showed no impact on L929 cell viability or cell death. Moreover, TiO2 NPs(1) exhibited a tendency to reduce H2O2-induced oxidative stress in L929 cells, while TiO2 NPs(2) had the opposite effect, likely due to their propensity for aggregation in the cell medium. Our findings suggest that the synthesized TiO2 NPs have potential as agents for modulating intracellular ROS levels at concentrations that do not compromise cell viability.

Název v anglickém jazyce

Effect of TiO2 Nanoparticles Defect Structure on their ROS Scavenging Ability

Popis výsledku anglicky

Titanium dioxide nanoparticles (TiO2 NPs) have attracted significant interest for their ability to modulate reactive oxygen species (ROS) levels within cells. These nanoparticles have shown promise in drug delivery, sonodynamic, photodynamic, photothermal, and ionizing radiation-based anti-cancer therapies, as well as antimicrobial and antioxidant applications. In this study, we analyzed the effects of TiO2 NP defect structures-specifically the presence of stoichiometric (Ti4+) and non-stoichiometric (Ti-3. and Ti-2.) titanium ions within the crystal lattice-and the aggregation state of TiO2 NPs on their ROS scavenging capabilities in both cell-free conditions and H2O2-treated L929 cells. Two types of TiO2 NPs with varying concentrations of Ti-3. and Ti-2. ions were synthesized and characterized using XRD, TEM, SAXS, and XPS techniques. The antioxidant properties of these TiO2 NPs were assessed through chemiluminescence and optical spectroscopy using ROS sensors. Results from chemiluminescence and total antioxidant capacity assays indicated that the synthesized TiO2 NPs possess radical scavenging abilities, with TiO2 NPs(1), containing a higher amount of non- stoichiometric titanium ions, demonstrating a stronger effect. Concentrations of up to 40 mg/L of TiO2 NPs showed no impact on L929 cell viability or cell death. Moreover, TiO2 NPs(1) exhibited a tendency to reduce H2O2-induced oxidative stress in L929 cells, while TiO2 NPs(2) had the opposite effect, likely due to their propensity for aggregation in the cell medium. Our findings suggest that the synthesized TiO2 NPs have potential as agents for modulating intracellular ROS levels at concentrations that do not compromise cell viability.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

2024 IEEE 14th International Conference Nanomaterials: Applications &amp; Properties (NAP)

ISBN

979-8-3503-8013-2

ISSN

—

e-ISSN

—

Počet stran výsledku

5

Strana od-do

1-5

Název nakladatele

IEEE

Místo vydání

New York

Místo konání akce

Riga

Datum konání akce

8. 9. 2024

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001424467800061