CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F05%3A00003842" target="_blank" >RIV/00216208:11120/05:00003842 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1634/stemcells.2004-0291" target="_blank" >http://dx.doi.org/10.1634/stemcells.2004-0291</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1634/stemcells.2004-0291" target="_blank" >10.1634/stemcells.2004-0291</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
Popis výsledku v původním jazyce
Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers. Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45(+) resident bone marrow-derived cells, whereas most cells were CD45-F-actin(+) keratocytes. A significant proportion (two thirds) of CD45(+) cells in the normal corneal stroma expressed CD34(+), whereas no CD45(-) c
Název v anglickém jazyce
CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
Popis výsledku anglicky
Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers. Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45(+) resident bone marrow-derived cells, whereas most cells were CD45-F-actin(+) keratocytes. A significant proportion (two thirds) of CD45(+) cells in the normal corneal stroma expressed CD34(+), whereas no CD45(-) c
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FF - ORL, oftalmologie, stomatologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2005
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Stem Cells
ISSN
1066-5099
e-ISSN
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Svazek periodika
23
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
507-515
Kód UT WoS článku
000228161800009
EID výsledku v databázi Scopus
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