Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma - Next step toward reliable non-invasive prenatal diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F10%3A00002619" target="_blank" >RIV/00216208:11120/10:00002619 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/10:6349

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma - Next step toward reliable non-invasive prenatal diagnostics

Popis výsledku v původním jazyce

We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF1A) and total (GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n = 51) or female (n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals (n = 29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF1A and SRY(? = 0.66, P < 0.001), total RASSF1A and GLO (? = 0.65,P < 0.001), SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A ratio (? = 0.62, P < 0.001) in maternal plasma. The results indicate that a universal fetal marker could be usef

Název v anglickém jazyce

Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma - Next step toward reliable non-invasive prenatal diagnostics

Popis výsledku anglicky

We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal (SRY and hypermethylated RASSF1A) and total (GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA (SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male (n = 51) or female (n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals (n = 29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF1A and SRY(? = 0.66, P < 0.001), total RASSF1A and GLO (? = 0.65,P < 0.001), SRY/GLO vs. hypermethylated RASSF1A/total RASSF1A ratio (? = 0.62, P < 0.001) in maternal plasma. The results indicate that a universal fetal marker could be usef

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental and Molecular Pathology

ISSN

0014-4800

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000284683300007

EID výsledku v databázi Scopus

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