Interlaboratory variability of CD34+stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F10%3A00002904" target="_blank" >RIV/00216208:11120/10:00002904 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/10:6329 RIV/00179906:_____/10:10084222 RIV/00064203:_____/10:6329 RIV/00023736:_____/10:00008922 a 2 dalších

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interlaboratory variability of CD34+stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic

Popis výsledku v původním jazyce

We present the results of a pilot study concerning the interlaboratory variability of CD34+ enumeration. Three surveys, each including a set of samples, were sent to participating Czech flow cytometry laboratories. The efficacy of this exercise was determined by the reduction in interlaboratory variation and the influence of method used on assay outcome. The variability in results of CD34+ enumeration declined with time. The mean coefficient of variation (CV) of measurement among laboratories dropped, from 58% in first survey to 32% in last survey. All tested variables (gating strategy, platform methodology, sample preparation) affected the variability of the assay. Sample preparation method was associated with a significant bias of absolute CD34+ cellcounts. Initially, the outcome of the measurement was also affected by the participating laboratory (identified by a unique laboratory number; ULN). However, laboratories with poorer performance modified their protocols during the study,

Název v anglickém jazyce

Interlaboratory variability of CD34+stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic

Popis výsledku anglicky

We present the results of a pilot study concerning the interlaboratory variability of CD34+ enumeration. Three surveys, each including a set of samples, were sent to participating Czech flow cytometry laboratories. The efficacy of this exercise was determined by the reduction in interlaboratory variation and the influence of method used on assay outcome. The variability in results of CD34+ enumeration declined with time. The mean coefficient of variation (CV) of measurement among laboratories dropped, from 58% in first survey to 32% in last survey. All tested variables (gating strategy, platform methodology, sample preparation) affected the variability of the assay. Sample preparation method was associated with a significant bias of absolute CD34+ cellcounts. Initially, the outcome of the measurement was also affected by the participating laboratory (identified by a unique laboratory number; ULN). However, laboratories with poorer performance modified their protocols during the study,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NR9268" target="_blank" >NR9268: Role adhezivních molekul a cytokinů v mobilizaci hemopoetických progenitorových buněk u zdravých dárců</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Laboratory Hematology

ISSN

1751-5521

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

6, Part

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000283595500006

EID výsledku v databázi Scopus

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