A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F11%3A00003424" target="_blank" >RIV/00216208:11120/11:00003424 - isvavai.cz</a>

Výsledek na webu

<a href="http://ar.iiarjournals.org/content/31/10/3125.full" target="_blank" >http://ar.iiarjournals.org/content/31/10/3125.full</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells

Popis výsledku v původním jazyce

Circulating tumor cells (CTCs) are of great importance for cancer diagnosis, prognosis and treatment. It is necessary to improve the ability to image and analyze them for their biological properties which determine their behavior in the patient. In the present study, using immunomagnetic beads, CTCs were rapidly isolated from the circulation of mice orthotopically implanted with human PC-3 prostate cancer cells stably expressing green fluorescent protein (GFP). The PC-3 GFP CTCs were then expanded in culture in parallel with the parental PC-3 GFP cell line. Both cell types were then inoculated onto the chorioallentoic membrane (CAM) of chick embryos. Eight days later, embryos were harvested and the brains were processed for frozen sections. The IV-100intravital laser scanning microscope enabled rapid identification of fluorescent metastatic foci within the chick embryonic brain. Inoculation of embryos with PC-3 GFP CTCs resulted in a 3 to 10-fold increase in brain metastasis when comp

Název v anglickém jazyce

A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells

Popis výsledku anglicky

Circulating tumor cells (CTCs) are of great importance for cancer diagnosis, prognosis and treatment. It is necessary to improve the ability to image and analyze them for their biological properties which determine their behavior in the patient. In the present study, using immunomagnetic beads, CTCs were rapidly isolated from the circulation of mice orthotopically implanted with human PC-3 prostate cancer cells stably expressing green fluorescent protein (GFP). The PC-3 GFP CTCs were then expanded in culture in parallel with the parental PC-3 GFP cell line. Both cell types were then inoculated onto the chorioallentoic membrane (CAM) of chick embryos. Eight days later, embryos were harvested and the brains were processed for frozen sections. The IV-100intravital laser scanning microscope enabled rapid identification of fluorescent metastatic foci within the chick embryonic brain. Inoculation of embryos with PC-3 GFP CTCs resulted in a 3 to 10-fold increase in brain metastasis when comp

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anticancer Research

ISSN

0250-7005

e-ISSN

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Svazek periodika

31

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

4

Strana od-do

3125-3128

Kód UT WoS článku

000295667700002

EID výsledku v databázi Scopus

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