Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F12%3A43900089" target="_blank" >RIV/00216208:11120/12:43900089 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/12:10106650 RIV/00064173:_____/12:43900089

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1582-4934.2011.01458.x" target="_blank" >http://dx.doi.org/10.1111/j.1582-4934.2011.01458.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1582-4934.2011.01458.x" target="_blank" >10.1111/j.1582-4934.2011.01458.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

Popis výsledku v původním jazyce

Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represent risk for HHC were examined), and in patients with iron deficiency anemia (IDA). Gene expressions of DMT1, ferroportin, Dcytb, hephaestin, HFE, and TFR1 were measured in duodenal biopsies using real-time PCRand Western blot. Serum hepcidin levels were measured using ELISA. DMT1, ferroportin, and TFR1 mRNA levels were significantly increased in post-phlebotomized hemochromatics relative to controls. mRNAs of all tested molecules were significantly increasedin patients with IDA compared to controls. The protein expression of ferroportin was increased in both groups of patients but not significantly. Spearman rank correlations showed that DMT1 vs. ferroportin, Dcytb vs. hephaestin and DMT1 v

Název v anglickém jazyce

Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

Popis výsledku anglicky

Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represent risk for HHC were examined), and in patients with iron deficiency anemia (IDA). Gene expressions of DMT1, ferroportin, Dcytb, hephaestin, HFE, and TFR1 were measured in duodenal biopsies using real-time PCRand Western blot. Serum hepcidin levels were measured using ELISA. DMT1, ferroportin, and TFR1 mRNA levels were significantly increased in post-phlebotomized hemochromatics relative to controls. mRNAs of all tested molecules were significantly increasedin patients with IDA compared to controls. The protein expression of ferroportin was increased in both groups of patients but not significantly. Spearman rank correlations showed that DMT1 vs. ferroportin, Dcytb vs. hephaestin and DMT1 v

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cellular and Molecular Medicine

ISSN

1582-1838

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1816-1826

Kód UT WoS článku

000306909200019

EID výsledku v databázi Scopus

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