2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F12%3A43906525" target="_blank" >RIV/00216208:11120/12:43906525 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-62703-029-8_2" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-029-8_2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-029-8_2" target="_blank" >10.1007/978-1-62703-029-8_2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

Popis výsledku v původním jazyce

Capillary electrophoresis (CE) with contactless conductivity detection (C4D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C4D determinationsof 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5 to 10 mol/L acetic acid. The time of analysis does not exceed 6 min., the limits of detection vary from 0.1 to 1.7 ?mol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal and amniotic fluid samples

Název v anglickém jazyce

2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

Popis výsledku anglicky

Capillary electrophoresis (CE) with contactless conductivity detection (C4D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C4D determinationsof 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5 to 10 mol/L acetic acid. The time of analysis does not exceed 6 min., the limits of detection vary from 0.1 to 1.7 ?mol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal and amniotic fluid samples

Druh

C - Kapitola v odborné knize

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F10%2F1231" target="_blank" >GAP206/10/1231: Nové elekroforetické přístupy k monitorování hladin neurotransmiterů bolesti v mozku vyšších živočichů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Clinical Applications of Capillary Electrophoresis: Methods and Protocols

ISBN

978-1-62703-028-1

Počet stran výsledku

11

Strana od-do

13-23

Počet stran knihy

311

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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