Very fast electrophoretic separation on commercial instruments using a combination of two capillaries with different internal diameters

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43906901" target="_blank" >RIV/00216208:11120/13:43906901 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/13:10133792

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201200524" target="_blank" >http://dx.doi.org/10.1002/elps.201200524</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201200524" target="_blank" >10.1002/elps.201200524</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Very fast electrophoretic separation on commercial instruments using a combination of two capillaries with different internal diameters

Popis výsledku v původním jazyce

A capillary formed by connecting a 9.7 cm long separation capillary with id 25 ?m with an auxiliary 22.9 cm long capillary with id 100 ?m (coupled capillary) was tested for electrophoretic separation at high electric field intensities. The coupled capillary was placed in the cassette of a standard electrophoresis apparatus. It was used in the short-end injection mode for separation of a mixture of dopamine, noradrenaline and adrenaline in a background electrolyte of 20 mM citric acid/NaOH, pH 3.2. An intensity of 2.7 kV cm(-1) was attained in the separation part of the capillary at a separation voltage of 30 kV, which is 2.9 times more than maximum intensity value attainable in a capillary with the same length with uniform id. At these high electric field intensities, the migration times of the tested neurotransmitters had values of 12.3 to 13.3 s and the attained separation efficiency was between 2350 and 2760 plates s(-1) . It is thus demonstrated that an effective separation instrum

Název v anglickém jazyce

Very fast electrophoretic separation on commercial instruments using a combination of two capillaries with different internal diameters

Popis výsledku anglicky

A capillary formed by connecting a 9.7 cm long separation capillary with id 25 ?m with an auxiliary 22.9 cm long capillary with id 100 ?m (coupled capillary) was tested for electrophoretic separation at high electric field intensities. The coupled capillary was placed in the cassette of a standard electrophoresis apparatus. It was used in the short-end injection mode for separation of a mixture of dopamine, noradrenaline and adrenaline in a background electrolyte of 20 mM citric acid/NaOH, pH 3.2. An intensity of 2.7 kV cm(-1) was attained in the separation part of the capillary at a separation voltage of 30 kV, which is 2.9 times more than maximum intensity value attainable in a capillary with the same length with uniform id. At these high electric field intensities, the migration times of the tested neurotransmitters had values of 12.3 to 13.3 s and the attained separation efficiency was between 2350 and 2760 plates s(-1) . It is thus demonstrated that an effective separation instrum

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

552-556

Kód UT WoS článku

000315169800011

EID výsledku v databázi Scopus

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