Pepsin Digest of Wheat Gliadin Fraction Increases Production of IL-1 beta via TLR4/MyD88/TRIF/MAPK/NF-kappa B Signaling Pathway and an NLRP3 Inflammasome Activation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43907500" target="_blank" >RIV/00216208:11120/13:43907500 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/13:00399914

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Pepsin Digest of Wheat Gliadin Fraction Increases Production of IL-1 beta via TLR4/MyD88/TRIF/MAPK/NF-kappa B Signaling Pathway and an NLRP3 Inflammasome Activation

Popis výsledku v původním jazyce

Celiac disease (CD) is a gluten-responsive, chronic inflammatory enteropathy. IL-1 cytokine family members IL-1 beta and IL-18 have been associated with the inflammatory conditions in CD patients. However, the mechanisms of IL-1 molecule activation in CDhave not yet been elucidated. We show in this study that peripheral blood mononuclear cells (PBMC) and monocytes from celiac patients responded to pepsin digest of wheat gliadin fraction (PDWGF) by a robust secretion of IL-1 beta and IL-1 alpha and a slightly elevated production of IL-18. The analysis of the upstream mechanisms underlying PDWGF-induced IL-1 beta production in celiac PBMC show that PDWGF-induced de novo pro-IL-1 beta synthesis, followed by a caspase-1 dependent processing and the secretion of mature IL-1 beta. This was promoted by K+ efflux and oxidative stress, and was independent of P2X7 receptor signaling. The PDWGF-induced IL-1 beta release was dependent on Nod-like receptor family containing pyrin domain 3 (NLRP3)

Název v anglickém jazyce

Pepsin Digest of Wheat Gliadin Fraction Increases Production of IL-1 beta via TLR4/MyD88/TRIF/MAPK/NF-kappa B Signaling Pathway and an NLRP3 Inflammasome Activation

Popis výsledku anglicky

Celiac disease (CD) is a gluten-responsive, chronic inflammatory enteropathy. IL-1 cytokine family members IL-1 beta and IL-18 have been associated with the inflammatory conditions in CD patients. However, the mechanisms of IL-1 molecule activation in CDhave not yet been elucidated. We show in this study that peripheral blood mononuclear cells (PBMC) and monocytes from celiac patients responded to pepsin digest of wheat gliadin fraction (PDWGF) by a robust secretion of IL-1 beta and IL-1 alpha and a slightly elevated production of IL-18. The analysis of the upstream mechanisms underlying PDWGF-induced IL-1 beta production in celiac PBMC show that PDWGF-induced de novo pro-IL-1 beta synthesis, followed by a caspase-1 dependent processing and the secretion of mature IL-1 beta. This was promoted by K+ efflux and oxidative stress, and was independent of P2X7 receptor signaling. The PDWGF-induced IL-1 beta release was dependent on Nod-like receptor family containing pyrin domain 3 (NLRP3)

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS One

ISSN

1932-6203

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

"e62426; 1"-11

Kód UT WoS článku

000321662800044

EID výsledku v databázi Scopus

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