Epinephrine-stimulated glycogen breakdown activates glycogen synthase and increases insulin-stimulated glucose uptake in epitrochlearis muscles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909450" target="_blank" >RIV/00216208:11120/15:43909450 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1152/ajpendo.00282.2014" target="_blank" >http://dx.doi.org/10.1152/ajpendo.00282.2014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1152/ajpendo.00282.2014" target="_blank" >10.1152/ajpendo.00282.2014</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Epinephrine-stimulated glycogen breakdown activates glycogen synthase and increases insulin-stimulated glucose uptake in epitrochlearis muscles

Popis výsledku v původním jazyce

Epinephrine increases glycogen synthase (GS) phosphorylation and decreases GS activity but also stimulates glycogen breakdown, and low glycogen content normally activates GS. To test the hypothesis that glycogen content directly regulates GS phosphorylation, glycogen breakdown was stimulated in condition with decreased GS activation. Saline or epinephrine (0.02 mg/100 g rat) was injected subcutaneously in Wistar rats (similar to 130 g) with low (24-h-fasted), normal (normal diet), and high glycogen content (fasted-refed), and epitrochlearis muscles were removed after 3 h and incubated ex vivo, eliminating epinephrine action. Epinephrine injection reduced glycogen content in epitrochlearis muscles with high (120.7 +/- 17.8 vs. 204.6 +/- 14.5 mmol/kg, P< 0.01) and normal glycogen (89.5 +/- 7.6 vs. 152 +/- 8.1 mmol/kg, P < 0.01), but not significantly in muscles with low glycogen (90.0 +/- 5.0 vs. 102.8 +/- 7.8 mmol/kg, P = 0.17). In saline-injected rats, GS phosphorylation at sites 2 +

Název v anglickém jazyce

Epinephrine-stimulated glycogen breakdown activates glycogen synthase and increases insulin-stimulated glucose uptake in epitrochlearis muscles

Popis výsledku anglicky

Epinephrine increases glycogen synthase (GS) phosphorylation and decreases GS activity but also stimulates glycogen breakdown, and low glycogen content normally activates GS. To test the hypothesis that glycogen content directly regulates GS phosphorylation, glycogen breakdown was stimulated in condition with decreased GS activation. Saline or epinephrine (0.02 mg/100 g rat) was injected subcutaneously in Wistar rats (similar to 130 g) with low (24-h-fasted), normal (normal diet), and high glycogen content (fasted-refed), and epitrochlearis muscles were removed after 3 h and incubated ex vivo, eliminating epinephrine action. Epinephrine injection reduced glycogen content in epitrochlearis muscles with high (120.7 +/- 17.8 vs. 204.6 +/- 14.5 mmol/kg, P< 0.01) and normal glycogen (89.5 +/- 7.6 vs. 152 +/- 8.1 mmol/kg, P < 0.01), but not significantly in muscles with low glycogen (90.0 +/- 5.0 vs. 102.8 +/- 7.8 mmol/kg, P = 0.17). In saline-injected rats, GS phosphorylation at sites 2 +

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

American Journal of Physiology - Endocrinology and Metabolism

ISSN

0193-1849

e-ISSN

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Svazek periodika

308

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

"E231"-"E240"

Kód UT WoS článku

000349303500006

EID výsledku v databázi Scopus

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