Development and validation of LC-MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909811" target="_blank" >RIV/00216208:11120/15:43909811 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/15:10312390 RIV/00023761:_____/15:#0000542 RIV/00064173:_____/15:#0000463

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.talanta.2015.03.013" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2015.03.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2015.03.013" target="_blank" >10.1016/j.talanta.2015.03.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development and validation of LC-MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid

Popis výsledku v původním jazyce

Bisphenol A (BPA) is a widely known endocrine disruptor with estrogenic, antiestrogenic or antiandrogenic properties. BPA could interfere with estrogen metabolism as well with receptor-mediated estrogen actions. Both environmental BPA and estrogens may be traced in body fluids, of which, besides the blood plasma, the seminal fluid is of particular interest regarding their possible interactions in the testis. The method for simultaneously determining BPA and estrogens is then needed, taking into account that their concentrations in these body fluid may differ. Here the method was developed and validated for measurements of BPA, estrone (E1), estradiol (E2) and estriol (E3) in blood plasma and seminal plasma using liquid chromatography-tandem mass spectrometry. Due to the phenolic moiety of all compounds, dansyl chloride derivatization could be used. The analytical criteria of the method with respect to expected concentration of the analytes were satisfactory. The lower limits of quantifications (LLOQ) amounted to 43.5, 4.0, 12.7, 6.7pg/mL for plasma BPA, E1, E2 and E3, and 28.9, 4.9, 4.5, 3.4pg/mL for seminal BPA, E1, E2 and E3, respectively. The concentrations of individual steroids differed between body fluids. To the best of our knowledge, this is the first method that enabled the measurement of estrogens and BPA together in one run. The concentrations of E1, E2 and for the first time also of E3 in seminal plasma in normospermic men are reported.

Název v anglickém jazyce

Development and validation of LC-MS/MS method for quantification of bisphenol A and estrogens in human plasma and seminal fluid

Popis výsledku anglicky

Bisphenol A (BPA) is a widely known endocrine disruptor with estrogenic, antiestrogenic or antiandrogenic properties. BPA could interfere with estrogen metabolism as well with receptor-mediated estrogen actions. Both environmental BPA and estrogens may be traced in body fluids, of which, besides the blood plasma, the seminal fluid is of particular interest regarding their possible interactions in the testis. The method for simultaneously determining BPA and estrogens is then needed, taking into account that their concentrations in these body fluid may differ. Here the method was developed and validated for measurements of BPA, estrone (E1), estradiol (E2) and estriol (E3) in blood plasma and seminal plasma using liquid chromatography-tandem mass spectrometry. Due to the phenolic moiety of all compounds, dansyl chloride derivatization could be used. The analytical criteria of the method with respect to expected concentration of the analytes were satisfactory. The lower limits of quantifications (LLOQ) amounted to 43.5, 4.0, 12.7, 6.7pg/mL for plasma BPA, E1, E2 and E3, and 28.9, 4.9, 4.5, 3.4pg/mL for seminal BPA, E1, E2 and E3, respectively. The concentrations of individual steroids differed between body fluids. To the best of our knowledge, this is the first method that enabled the measurement of estrogens and BPA together in one run. The concentrations of E1, E2 and for the first time also of E3 in seminal plasma in normospermic men are reported.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

—

Projekt

<a href="/cs/project/NT13369" target="_blank" >NT13369: Vliv vybraných endokrinních disruptorů na lidskou spermatogenezi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

140

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

62-67

Kód UT WoS článku

000356987400010

EID výsledku v databázi Scopus

2-s2.0-84925679903