Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909891" target="_blank" >RIV/00216208:11120/15:43909891 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/15:00452051
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.envres.2015.06.046" target="_blank" >http://dx.doi.org/10.1016/j.envres.2015.06.046</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envres.2015.06.046" target="_blank" >10.1016/j.envres.2015.06.046</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells
Popis výsledku v původním jazyce
Pollution of the environment represents one of less explored potential reasons for the worldwide epidemic of type 2 diabetes. One of the most prevalent organochlorine pollutants remains the pesticide DDT and its degradation product DDE. Despite some epidemiologic correlations between levels of DDT and DDE in human organism and the prevalence of diabetes, there is almost no information about the exact targets of these compounds inside pancreatic beta cells. To detect functional areas of pancreatic beta cells that could be affected by exposure to DDT and DDE, we analyzed changes in protein expression in the NES2Y human pancreatic beta cell line exposed to three sublethal concentrations (0.1?M, 1?M, 10?M) of DDT and DDE for 1 month. Protein separation andidentification was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectrometry. With these techniques, four proteins were found downregulated after exposure to 10?M DDT: three cytoskeletal proteins (cytoker
Název v anglickém jazyce
Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells
Popis výsledku anglicky
Pollution of the environment represents one of less explored potential reasons for the worldwide epidemic of type 2 diabetes. One of the most prevalent organochlorine pollutants remains the pesticide DDT and its degradation product DDE. Despite some epidemiologic correlations between levels of DDT and DDE in human organism and the prevalence of diabetes, there is almost no information about the exact targets of these compounds inside pancreatic beta cells. To detect functional areas of pancreatic beta cells that could be affected by exposure to DDT and DDE, we analyzed changes in protein expression in the NES2Y human pancreatic beta cell line exposed to three sublethal concentrations (0.1?M, 1?M, 10?M) of DDT and DDE for 1 month. Protein separation andidentification was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectrometry. With these techniques, four proteins were found downregulated after exposure to 10?M DDT: three cytoskeletal proteins (cytoker
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DN - Vliv životního prostředí na zdraví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Environmental Research
ISSN
0013-9351
e-ISSN
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Svazek periodika
142
Číslo periodika v rámci svazku
October
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
257-263
Kód UT WoS článku
000363602800031
EID výsledku v databázi Scopus
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