Differences in promoter DNA methylation and mRNA expression of individual alleles of the HLA class II DQA1 gene
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910218" target="_blank" >RIV/00216208:11120/15:43910218 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.imlet.2015.08.006" target="_blank" >http://dx.doi.org/10.1016/j.imlet.2015.08.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.imlet.2015.08.006" target="_blank" >10.1016/j.imlet.2015.08.006</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Differences in promoter DNA methylation and mRNA expression of individual alleles of the HLA class II DQA1 gene
Popis výsledku v původním jazyce
Objectives: Extensive polymorphism of HLA class II genes is not restricted to the coding region of the gene. It extends also to the linked promoter region, where it forms the basis for different levels of individual allele's expression. Differential expression of HLA class II alleles can shape an immune response and influence the risk of developing autoimmune disease. In addition to genetic variability, variation in epigenetic modifications, including DNA methylation, can be another cause of the unevenexpression of individual alleles. We aimed to analyze the DNA methylation of promoter sequences and the levels of expression of individual DQA1 gene alleles, interallelic variation of these two characteristics and the relationship between them. Methods:The 60 healthy donors included into study were HLA-DRB1, HLA-DQB1 and HLA-DQA1 genotyped using PCR-SSP. Genomic DNA was treated by sodium bisulfite and the target segment in the HLA-DQA1 gene promoter was PCR amplified. PCR product was cl
Název v anglickém jazyce
Differences in promoter DNA methylation and mRNA expression of individual alleles of the HLA class II DQA1 gene
Popis výsledku anglicky
Objectives: Extensive polymorphism of HLA class II genes is not restricted to the coding region of the gene. It extends also to the linked promoter region, where it forms the basis for different levels of individual allele's expression. Differential expression of HLA class II alleles can shape an immune response and influence the risk of developing autoimmune disease. In addition to genetic variability, variation in epigenetic modifications, including DNA methylation, can be another cause of the unevenexpression of individual alleles. We aimed to analyze the DNA methylation of promoter sequences and the levels of expression of individual DQA1 gene alleles, interallelic variation of these two characteristics and the relationship between them. Methods:The 60 healthy donors included into study were HLA-DRB1, HLA-DQB1 and HLA-DQA1 genotyped using PCR-SSP. Genomic DNA was treated by sodium bisulfite and the target segment in the HLA-DQA1 gene promoter was PCR amplified. PCR product was cl
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EC - Imunologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Immunology Letters
ISSN
0165-2478
e-ISSN
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Svazek periodika
167
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
8
Strana od-do
147-154
Kód UT WoS článku
000362628200012
EID výsledku v databázi Scopus
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