The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910347" target="_blank" >RIV/00216208:11120/15:43910347 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/15:10321964 RIV/61388971:_____/15:00462022

Výsledek na webu

<a href="http://dx.doi.org/10.1515/cmble-2015-0054" target="_blank" >http://dx.doi.org/10.1515/cmble-2015-0054</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/cmble-2015-0054" target="_blank" >10.1515/cmble-2015-0054</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

Popis výsledku v původním jazyce

Experiments using cultured primary cells or cell lines are a routine in vitro approach used across multiple biological disciplines, However, the structural and functional influences of various cultureware materials on cultured cells is not clearly understood. Surface treatments of cultureware have proven to have profound effects on cell viability and proliferation. In this study, we investigated the impact of polystyrene and fluorocarbon cultureware dishes on the proteomic profile of differentiated 3T3-L1 preadipocytes. After expansion and differentiation of cells on appropriate cultureware dishes, cell lysates were separated using two-dimensional gel electrophoresis and proteins were visualized with Coomassie blue staining. Spots with the highest differential expression between the two culture conditions were subsequently analyzed using matrix-assisted laser desorption/ionization mass spectrometry and the identified proteins were subjected to pathway analysis. We observed that 43% of all spots were differentially expressed depending on the cultureware. Pathway analysis revealed that glucose metabolism, mitochondrial structure and cell differentiation, represented by 14-3-3 protein-mediated signaling and the mitochondrial inner membrane organizing system (MINOS), were significantly affected by cultureware material. These results indicate that cultureware material can have a profound effect on key adipocyte functional pathways. These effects modifications of the cells should be reflected in the design of in vitro experiments and interpretation of their results.

Název v anglickém jazyce

The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes

Popis výsledku anglicky

Experiments using cultured primary cells or cell lines are a routine in vitro approach used across multiple biological disciplines, However, the structural and functional influences of various cultureware materials on cultured cells is not clearly understood. Surface treatments of cultureware have proven to have profound effects on cell viability and proliferation. In this study, we investigated the impact of polystyrene and fluorocarbon cultureware dishes on the proteomic profile of differentiated 3T3-L1 preadipocytes. After expansion and differentiation of cells on appropriate cultureware dishes, cell lysates were separated using two-dimensional gel electrophoresis and proteins were visualized with Coomassie blue staining. Spots with the highest differential expression between the two culture conditions were subsequently analyzed using matrix-assisted laser desorption/ionization mass spectrometry and the identified proteins were subjected to pathway analysis. We observed that 43% of all spots were differentially expressed depending on the cultureware. Pathway analysis revealed that glucose metabolism, mitochondrial structure and cell differentiation, represented by 14-3-3 protein-mediated signaling and the mitochondrial inner membrane organizing system (MINOS), were significantly affected by cultureware material. These results indicate that cultureware material can have a profound effect on key adipocyte functional pathways. These effects modifications of the cells should be reflected in the design of in vitro experiments and interpretation of their results.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA13-27735S" target="_blank" >GA13-27735S: Poruchy v metabolismu tukové tkáně vedoucí k rozvoji diabetu u syndromu spánkové apnoe (Studie SAMET - spánková apnoe a metabolismus tukové tkáně)</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular &amp; Molecular Biology Letters

ISSN

1425-8153

e-ISSN

—

Svazek periodika

20

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

919-936

Kód UT WoS článku

000372089200013

EID výsledku v databázi Scopus

2-s2.0-84962620272