Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910511" target="_blank" >RIV/00216208:11120/15:43910511 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/15:#0000495

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s13054-015-1160-x" target="_blank" >http://dx.doi.org/10.1186/s13054-015-1160-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13054-015-1160-x" target="_blank" >10.1186/s13054-015-1160-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness

Popis výsledku v původním jazyce

BACKGROUND: Mitochondrial damage occurs in the acute phase of critical illness, followed by activation of mitochondrial biogenesis in survivors. It has been hypothesized that bioenergetics failure of skeletal muscle may contribute to the development of ICU-acquired weakness. The aim of the present study was to determine whether mitochondrial dysfunction persists until protracted phase of critical illness. METHODS: In this single-centre controlled-cohort ex vivo proof-of-concept pilot study, we obtainedvastus lateralis biopsies from ventilated patients with ICU-acquired weakness (n = 8) and from age and sex-matched metabolically healthy controls (n = 8). Mitochondrial functional indices were measured in cytosolic context by high-resolution respirometryin tissue homogenates, activities of respiratory complexes by spectrophotometry and individual functional capacities were correlated with concentrations of electron transport chain key subunits from respiratory complexes II, III, IV and

Název v anglickém jazyce

Mitochondrial function in skeletal muscle of patients with protracted critical illness and ICU-acquired weakness

Popis výsledku anglicky

BACKGROUND: Mitochondrial damage occurs in the acute phase of critical illness, followed by activation of mitochondrial biogenesis in survivors. It has been hypothesized that bioenergetics failure of skeletal muscle may contribute to the development of ICU-acquired weakness. The aim of the present study was to determine whether mitochondrial dysfunction persists until protracted phase of critical illness. METHODS: In this single-centre controlled-cohort ex vivo proof-of-concept pilot study, we obtainedvastus lateralis biopsies from ventilated patients with ICU-acquired weakness (n = 8) and from age and sex-matched metabolically healthy controls (n = 8). Mitochondrial functional indices were measured in cytosolic context by high-resolution respirometryin tissue homogenates, activities of respiratory complexes by spectrophotometry and individual functional capacities were correlated with concentrations of electron transport chain key subunits from respiratory complexes II, III, IV and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FI - Traumatologie a ortopedie

OECD FORD obor

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Projekt

<a href="/cs/project/NT12319" target="_blank" >NT12319: Multiparametrická charakteristika metabolismu lidských myoblastů ve vztahu k patogenezi svalové slabosti kriticky nemocných</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Critical Care

ISSN

1466-609X

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

"ID 448"

Kód UT WoS článku

000367057400001

EID výsledku v databázi Scopus

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