The use of polarity switching for the sensitive determination of nitrate in human cerebrospinal fluid by capillary electrophoresis with contactless conductivity detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F16%3A43911348" target="_blank" >RIV/00216208:11120/16:43911348 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.038" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2016.04.038</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2016.04.038" target="_blank" >10.1016/j.chroma.2016.04.038</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The use of polarity switching for the sensitive determination of nitrate in human cerebrospinal fluid by capillary electrophoresis with contactless conductivity detection
Popis výsledku v původním jazyce
A new electrophoretic stacking method has been developed for the sensitive determination of nitrates in cerebrospinal fluid. 2M acetic acid was used as the BGE; inorganic anions were detected using a contactless conductivity detector and separation was carried out in an INST-coated capillary with inner diameter of 25μm. The sample of cerebrospinal fluid was injected in a large volume into the short end of the separation capillary (15cm) and separation first occurred in the isotachophoretic mode, where a long zone of the majority chloride migrates in the capillary and is followed by a concentrated zone of the unseparated nitrates. The sample zone passes to the end of the capillary where more than 99% of the chlorides are let out. Then the polarity of the voltage is switched and separation occurs in the zone electrophoresis mode, in which the nitrates are separated from the zone of chlorides. The time of switching the polarity is determined by the decrease in the electrophoretic current. Up to 99.95% of the original amount of chlorides present in the cerebrospinal fluid could be let out of the capillary by this technique, thus increasing the signal/noise ratio by up to 60-fold compared to classical electrophoretic separation.
Název v anglickém jazyce
The use of polarity switching for the sensitive determination of nitrate in human cerebrospinal fluid by capillary electrophoresis with contactless conductivity detection
Popis výsledku anglicky
A new electrophoretic stacking method has been developed for the sensitive determination of nitrates in cerebrospinal fluid. 2M acetic acid was used as the BGE; inorganic anions were detected using a contactless conductivity detector and separation was carried out in an INST-coated capillary with inner diameter of 25μm. The sample of cerebrospinal fluid was injected in a large volume into the short end of the separation capillary (15cm) and separation first occurred in the isotachophoretic mode, where a long zone of the majority chloride migrates in the capillary and is followed by a concentrated zone of the unseparated nitrates. The sample zone passes to the end of the capillary where more than 99% of the chlorides are let out. Then the polarity of the voltage is switched and separation occurs in the zone electrophoresis mode, in which the nitrates are separated from the zone of chlorides. The time of switching the polarity is determined by the decrease in the electrophoretic current. Up to 99.95% of the original amount of chlorides present in the cerebrospinal fluid could be let out of the capillary by this technique, thus increasing the signal/noise ratio by up to 60-fold compared to classical electrophoretic separation.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/GA15-03139S" target="_blank" >GA15-03139S: Nové elektroforetické přístupy pro studium obezity a diabetu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
—
Svazek periodika
1447
Číslo periodika v rámci svazku
May
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
148-154
Kód UT WoS článku
000375888600018
EID výsledku v databázi Scopus
2-s2.0-84981764243