Comparison of early (2 days) and later (28 days) response of adipose tissue to very-low calorie diet in obese women

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F16%3A43912251" target="_blank" >RIV/00216208:11120/16:43912251 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1210/jc.2016-2161" target="_blank" >http://dx.doi.org/10.1210/jc.2016-2161</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1210/jc.2016-2161" target="_blank" >10.1210/jc.2016-2161</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of early (2 days) and later (28 days) response of adipose tissue to very-low calorie diet in obese women

Popis výsledku v původním jazyce

CONTEXT: Beneficial metabolic effects of calorie restriction found in the early stage of hypocalorie diets may be caused by the modulation of metabolic and endocrine function of adipose tissue. OBJECTIVE: To compare metabolic and inflammation-related characteristics of subcutaneous adipose tissue (SAAT) in the early (2 days) and later (28 days) phase of VLCD. Design, Setting, Intervention and Patients: 17 moderately obese premenopausal women followed 800 kcal/d VLCD for 28 days. Anthropometric measurements, blood sampling and biopsy of SAAT were performed before diet and after 2 and 28 days of VLCD. MAIN OUTCOME MEASURE(S): mRNA expression of 50 genes related to lipid metabolism, inflammation, and fibrosis were analyzed in SAAT. Secretion of adipokines was determined in SAAT explants and adipokines, FGF21 and CRP were measured in plasma. RESULTS: In the early phase of VLCD expression of lipolytic genes was increased while expression of lipogenic genes was significantly suppressed. The inflammatory markers in SAAT remained unchanged. At the later phase, expression of genes involved in lipogenesis and β-oxidation was markedly suppressed, while expression of inflammatory markers was increased. The changes of lipogenic genes after 28 days of VLCD correlated with FGF21 changes. CONCLUSION: The early and later phases of VLCD differ in respect to metabolic and inflammatory responses in SAAT. The expression changes in SAAT in the early phase of VLCD could not explain the effect of short calorie restriction on the improvement of insulin sensitivity. An interplay of SAAT with liver function during VLCD mediated by FGF21 might be suggested.

Název v anglickém jazyce

Comparison of early (2 days) and later (28 days) response of adipose tissue to very-low calorie diet in obese women

Popis výsledku anglicky

CONTEXT: Beneficial metabolic effects of calorie restriction found in the early stage of hypocalorie diets may be caused by the modulation of metabolic and endocrine function of adipose tissue. OBJECTIVE: To compare metabolic and inflammation-related characteristics of subcutaneous adipose tissue (SAAT) in the early (2 days) and later (28 days) phase of VLCD. Design, Setting, Intervention and Patients: 17 moderately obese premenopausal women followed 800 kcal/d VLCD for 28 days. Anthropometric measurements, blood sampling and biopsy of SAAT were performed before diet and after 2 and 28 days of VLCD. MAIN OUTCOME MEASURE(S): mRNA expression of 50 genes related to lipid metabolism, inflammation, and fibrosis were analyzed in SAAT. Secretion of adipokines was determined in SAAT explants and adipokines, FGF21 and CRP were measured in plasma. RESULTS: In the early phase of VLCD expression of lipolytic genes was increased while expression of lipogenic genes was significantly suppressed. The inflammatory markers in SAAT remained unchanged. At the later phase, expression of genes involved in lipogenesis and β-oxidation was markedly suppressed, while expression of inflammatory markers was increased. The changes of lipogenic genes after 28 days of VLCD correlated with FGF21 changes. CONCLUSION: The early and later phases of VLCD differ in respect to metabolic and inflammatory responses in SAAT. The expression changes in SAAT in the early phase of VLCD could not explain the effect of short calorie restriction on the improvement of insulin sensitivity. An interplay of SAAT with liver function during VLCD mediated by FGF21 might be suggested.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of Clinical Endocrinology &amp; Metabolism

ISSN

0021-972X

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

5021-5029

Kód UT WoS článku

000390951000058

EID výsledku v databázi Scopus

2-s2.0-85003480081