Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F18%3A43916955" target="_blank" >RIV/00216208:11120/18:43916955 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064190:_____/18:N0000041 RIV/00216208:11110/18:10377541 RIV/00064165:_____/18:10377541

Výsledek na webu

<a href="https://doi.org/10.1155/2018/1975056" target="_blank" >https://doi.org/10.1155/2018/1975056</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2018/1975056" target="_blank" >10.1155/2018/1975056</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns

Popis výsledku v původním jazyce

Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates. Thirteen newborns on ECMO and 13 healthy newborns were included in the study. Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex (R) xMAP multianalyte profiling platform. The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry. The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1). Inflammatory markers (procalcitonin, IL-1 beta, IL-6, and IL-22) were increased in the ECMO group (P &lt; 0 01). The assessment of endothelial markers showed higher concentrations of endocan and angiopoietin-2 (P &lt; 0 01) in the ECMO group while VEGF in the ECMO group was significantly lower (P &lt; 0 01). In the ECMO group, the concentration of annexin-V-positive microvesicles (total microvesicles) and endothelial microvesicles expressing mucosal vascular addressin cell adhesion molecule 1 (MadCAM1) was increased (P = 0 05). In summary, we found increased concentrations of soluble inflammatory and endothelial markers in the plasma of critically ill newborns with multiple organ dysfunction. Increased plasma concentrations of microvesicles may reflect the activation or damage of blood cells and vasculature including endothelial cells. The measurement of cell membrane-derived microvesicles may be added to the panel of established inflammatory markers in order to increase the sensitivity and specificity of the diagnostic process in critically ill newborns.

Název v anglickém jazyce

Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns

Popis výsledku anglicky

Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates. Thirteen newborns on ECMO and 13 healthy newborns were included in the study. Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex (R) xMAP multianalyte profiling platform. The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry. The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1). Inflammatory markers (procalcitonin, IL-1 beta, IL-6, and IL-22) were increased in the ECMO group (P &lt; 0 01). The assessment of endothelial markers showed higher concentrations of endocan and angiopoietin-2 (P &lt; 0 01) in the ECMO group while VEGF in the ECMO group was significantly lower (P &lt; 0 01). In the ECMO group, the concentration of annexin-V-positive microvesicles (total microvesicles) and endothelial microvesicles expressing mucosal vascular addressin cell adhesion molecule 1 (MadCAM1) was increased (P = 0 05). In summary, we found increased concentrations of soluble inflammatory and endothelial markers in the plasma of critically ill newborns with multiple organ dysfunction. Increased plasma concentrations of microvesicles may reflect the activation or damage of blood cells and vasculature including endothelial cells. The measurement of cell membrane-derived microvesicles may be added to the panel of established inflammatory markers in order to increase the sensitivity and specificity of the diagnostic process in critically ill newborns.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/NV16-27800A" target="_blank" >NV16-27800A: Poškození endotelu u novorozenců: diagnostický význam biomarkerů a mikropartikulí u onemocnění ovlivňujících novorozeneckou mortalitu a morbiditu</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mediators of Inflammation

ISSN

0962-9351

e-ISSN

—

Svazek periodika

2018

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

"Article 1975056"

Kód UT WoS článku

000440502100001

EID výsledku v databázi Scopus

2-s2.0-85058699386