Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917764" target="_blank" >RIV/00216208:11120/19:43917764 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-4939-9213-3_2" target="_blank" >https://doi.org/10.1007/978-1-4939-9213-3_2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-9213-3_2" target="_blank" >10.1007/978-1-4939-9213-3_2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

Popis výsledku v původním jazyce

Capillary electrophoresis (CE) with contactless conductivity detection (C 4 D) represents a strong tool for determining amino acids in clinical samples. This chapter provides detailed instructions for CE/C 4 D determination of the branched-chain amino acids (BCAAs) valine, isoleucine, and leucine in human plasma, which can be readily employed in physiological studies. Baseline separation of all the BCAAs is achieved on a short separation length equal to 18 cm in optimized background electrolyte consisting of 3.2 M acetic acid dissolved in 20% v/v methanol with addition of 1.0% v/v INST-coating solution. The analysis time does not exceed 3 min and the limit of detection is 0.4 μM for all BCAAs. The pretreatment of human plasma is very simple and is based on fourfold plasma dilution by acetonitrile and subsequent filtration. Only 50 μL of plasma is used for the analysis. The high sensitivity of the CE/C 4 D method is achieved by injecting a large volume of sample, combined with application of negative pressure to flush the acetonitrile zone out of the capillary.

Název v anglickém jazyce

Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

Popis výsledku anglicky

Capillary electrophoresis (CE) with contactless conductivity detection (C 4 D) represents a strong tool for determining amino acids in clinical samples. This chapter provides detailed instructions for CE/C 4 D determination of the branched-chain amino acids (BCAAs) valine, isoleucine, and leucine in human plasma, which can be readily employed in physiological studies. Baseline separation of all the BCAAs is achieved on a short separation length equal to 18 cm in optimized background electrolyte consisting of 3.2 M acetic acid dissolved in 20% v/v methanol with addition of 1.0% v/v INST-coating solution. The analysis time does not exceed 3 min and the limit of detection is 0.4 μM for all BCAAs. The pretreatment of human plasma is very simple and is based on fourfold plasma dilution by acetonitrile and subsequent filtration. Only 50 μL of plasma is used for the analysis. The high sensitivity of the CE/C 4 D method is achieved by injecting a large volume of sample, combined with application of negative pressure to flush the acetonitrile zone out of the capillary.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA18-04902S" target="_blank" >GA18-04902S: Instrumentace pro kontinuální on-line elektroforetické monitorování metabolických procesů v živých organismech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Clinical Applications of Capillary Electrophoresis: Methods and Protocols

ISBN

978-1-4939-9212-6

Počet stran výsledku

10

Strana od-do

15-24

Počet stran knihy

273

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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