Electromembrane extraction of anesthetic ketamine on-line coupled to capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F23%3A43925753" target="_blank" >RIV/00216208:11120/23:43925753 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10464911

Výsledek na webu

<a href="https://doi.org/10.1016/j.microc.2023.108886" target="_blank" >https://doi.org/10.1016/j.microc.2023.108886</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2023.108886" target="_blank" >10.1016/j.microc.2023.108886</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electromembrane extraction of anesthetic ketamine on-line coupled to capillary electrophoresis

Popis výsledku v původním jazyce

The anesthetic ketamine is extracted from the sample solutions by electromembrane extraction on-line coupled with capillary electrophoresis (CE) at a short separation path. Ketamine is extracted from model acidic and alkaline aqueous solutions, or from untreated human urine as donor into an acidic acceptor solution, which acts also as background electrolyte for CE. Extraction occurs in a flow-through extraction mini probe with an internal volume of 1.1 µL, consisting of a porous hollow fiber with pores filled with nitrophenyloctyl ether as extraction liquid. The probe is immersed in a 1.5 mL vial of analyte solution, the vial is vibrationally agitated during extraction, and ketamine is extracted at 150 V for 300 s. It was found that the pH of the sample solution does not affect the extraction efficiency, i.e., it does not depend on whether ketamine is in the protonated form or as a neutral molecule during extraction; the enrichment factor is 16-19, the recovery factor 1.5 %. The calibration dependence is linear in both model samples in the concentration range 2 to 50 µM and in urine in the concentration range 2.5 to 10 µM with the same slope in both sample types. The LOD values are around 1 µM. The extraction probe is reusable. However, between runs a purification process with the probe immersed in the background electrolyte is necessary to remove ketamine trapped in the membrane liquid.

Název v anglickém jazyce

Electromembrane extraction of anesthetic ketamine on-line coupled to capillary electrophoresis

Popis výsledku anglicky

The anesthetic ketamine is extracted from the sample solutions by electromembrane extraction on-line coupled with capillary electrophoresis (CE) at a short separation path. Ketamine is extracted from model acidic and alkaline aqueous solutions, or from untreated human urine as donor into an acidic acceptor solution, which acts also as background electrolyte for CE. Extraction occurs in a flow-through extraction mini probe with an internal volume of 1.1 µL, consisting of a porous hollow fiber with pores filled with nitrophenyloctyl ether as extraction liquid. The probe is immersed in a 1.5 mL vial of analyte solution, the vial is vibrationally agitated during extraction, and ketamine is extracted at 150 V for 300 s. It was found that the pH of the sample solution does not affect the extraction efficiency, i.e., it does not depend on whether ketamine is in the protonated form or as a neutral molecule during extraction; the enrichment factor is 16-19, the recovery factor 1.5 %. The calibration dependence is linear in both model samples in the concentration range 2 to 50 µM and in urine in the concentration range 2.5 to 10 µM with the same slope in both sample types. The LOD values are around 1 µM. The extraction probe is reusable. However, between runs a purification process with the probe immersed in the background electrolyte is necessary to remove ketamine trapped in the membrane liquid.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA22-22398S" target="_blank" >GA22-22398S: Mikrofluidní a elektronická zařízení pro on-line elektroforetickou analýzu tukové tkáně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

191

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

108886

Kód UT WoS článku

001008669600001

EID výsledku v databázi Scopus

2-s2.0-85160063051