Caffeic acid does not protect human pancreatic β cells against stearic acid-induced ER stress and apoptosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F24%3A43926915" target="_blank" >RIV/00216208:11120/24:43926915 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/ejlt.202300206" target="_blank" >https://doi.org/10.1002/ejlt.202300206</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ejlt.202300206" target="_blank" >10.1002/ejlt.202300206</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Caffeic acid does not protect human pancreatic β cells against stearic acid-induced ER stress and apoptosis

Popis výsledku v původním jazyce

Long-term elevation of saturated fatty acids in blood has a deleterious effect on pancreatic beta-cell function and survival, leading to endoplasmic reticulum (ER) stress and apoptosis. This fundamentally contributes to type 2 diabetes development. Caffeic acid (CA) was found to protect various cell types against several proapoptotic stimuli, including fatty acids. However, its potential protective effect against fatty acid-induced apoptosis was not ascertained in pancreatic beta cells yet. Therefore, the objective of this study was to examine this in the human pancreatic beta-cell lines NES2Y and 1.1B4. In both cell lines, CA did not modify the effect of saturated stearic acid (SA) on beta-cell growth and viability. At higher concentrations, CA significantly even intensified the adverse effect of SA. Consistent with this, CA did not exhibit any inhibitory effect on SA-induced markers of ongoing apoptosis as well as ER stress. At higher concentrations, CA again slightly potentiated the effect of SA. CA applied alone was well tolerated up to 1 mM; however, at higher concentrations, it had detrimental effects in both cell lines. To conclude, we have shown that the treatment with caffeic acid has no inhibitory effect on SA-induced ER stress and apoptosis in the human pancreatic beta cells. Moreover, at higher concentrations, CA has proapoptotic potential.

Název v anglickém jazyce

Caffeic acid does not protect human pancreatic β cells against stearic acid-induced ER stress and apoptosis

Popis výsledku anglicky

Long-term elevation of saturated fatty acids in blood has a deleterious effect on pancreatic beta-cell function and survival, leading to endoplasmic reticulum (ER) stress and apoptosis. This fundamentally contributes to type 2 diabetes development. Caffeic acid (CA) was found to protect various cell types against several proapoptotic stimuli, including fatty acids. However, its potential protective effect against fatty acid-induced apoptosis was not ascertained in pancreatic beta cells yet. Therefore, the objective of this study was to examine this in the human pancreatic beta-cell lines NES2Y and 1.1B4. In both cell lines, CA did not modify the effect of saturated stearic acid (SA) on beta-cell growth and viability. At higher concentrations, CA significantly even intensified the adverse effect of SA. Consistent with this, CA did not exhibit any inhibitory effect on SA-induced markers of ongoing apoptosis as well as ER stress. At higher concentrations, CA again slightly potentiated the effect of SA. CA applied alone was well tolerated up to 1 mM; however, at higher concentrations, it had detrimental effects in both cell lines. To conclude, we have shown that the treatment with caffeic acid has no inhibitory effect on SA-induced ER stress and apoptosis in the human pancreatic beta cells. Moreover, at higher concentrations, CA has proapoptotic potential.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Lipid Science and Technology

ISSN

1438-7697

e-ISSN

1438-9312

Svazek periodika

126

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

2300206

Kód UT WoS článku

001197065700001

EID výsledku v databázi Scopus

2-s2.0-85190394651