Metallothioneins and Cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5411" target="_blank" >RIV/00216208:11130/09:5411 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/09:5411

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Metallothioneins and Cancer

Popis výsledku v původním jazyce

Metallothionein (MT) is a protein playing role in homeostasis of metal ions. Its relation with formation and progression of tumors is discussed. Here, we propose a new methodological approach for visualization of MT on PVDF membranes after dot- and electroblotting by using a commercial monoclonal antibody and polyclonalchicken antibodies. The optimized procedure was as follows. We dotted sample on membrane and let it to dry. Then, we blocked the membrane with BSA. After that, the membrane was incubatedin chicken primary antibody, washed, and incubated in secondary antibody conjugated with horseradish peroxidase. To visualize, we used 3-aminoethyl-9-carbazole. Under these conditions, we estimated detection limit as 3 pg of MT per 1 microL. The optimalapproach was further utilized for detection of MT level in 2 human fibroblast lines and in serum obtained from children with medulloblastoma. The results were in good agreement with differential pulse voltammetry ?Brdicka reaction.

Název v anglickém jazyce

Metallothioneins and Cancer

Popis výsledku anglicky

Metallothionein (MT) is a protein playing role in homeostasis of metal ions. Its relation with formation and progression of tumors is discussed. Here, we propose a new methodological approach for visualization of MT on PVDF membranes after dot- and electroblotting by using a commercial monoclonal antibody and polyclonalchicken antibodies. The optimized procedure was as follows. We dotted sample on membrane and let it to dry. Then, we blocked the membrane with BSA. After that, the membrane was incubatedin chicken primary antibody, washed, and incubated in secondary antibody conjugated with horseradish peroxidase. To visualize, we used 3-aminoethyl-9-carbazole. Under these conditions, we estimated detection limit as 3 pg of MT per 1 microL. The optimalapproach was further utilized for detection of MT level in 2 human fibroblast lines and in serum obtained from children with medulloblastoma. The results were in good agreement with differential pulse voltammetry ?Brdicka reaction.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA401990701" target="_blank" >IAA401990701: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Protein & Peptide Science

ISSN

1389-2037

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

16

Strana od-do

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Kód UT WoS článku

000268918200004

EID výsledku v databázi Scopus

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