Effect of Culture Substrate and Culture Conditions on Lens Epithelial Cell Proliferation and alpha-smooth Muscle Actin Expression

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F09%3A5741" target="_blank" >RIV/00216208:11130/09:5741 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Culture Substrate and Culture Conditions on Lens Epithelial Cell Proliferation and alpha-smooth Muscle Actin Expression

Popis výsledku v původním jazyce

The most common complication following cataract surgery is the posterior capsule opacification (PCO). It is the manifestation of migration and proliferation of lens epithelial cells (LEC). We used the primary and secondary culture of porcine LECs cultivated on collagen I, IV, glass, and uncoated plastic dishes. We studied cell proliferation, and expression of ? -SMA, and of alpha, beta, and gamma-crystallins. The effect of medium exchange schedule was studied using the TOTL-86 rabbit epithelial lens cell line. There was no difference in growth characteristics of primary cultures on different substrates. In secondary culture, LECs adhered better on the collagen surfaces. The culture substrate influenced the LEC proliferation and ?-SMA expression. The proliferation was enhanced when the medium was changed in comparison with an extra medium added on the 4th day. The cells did not synthesize ?- or ?-crystalline, the expression of ?-crystallin diminished.

Název v anglickém jazyce

Effect of Culture Substrate and Culture Conditions on Lens Epithelial Cell Proliferation and alpha-smooth Muscle Actin Expression

Popis výsledku anglicky

The most common complication following cataract surgery is the posterior capsule opacification (PCO). It is the manifestation of migration and proliferation of lens epithelial cells (LEC). We used the primary and secondary culture of porcine LECs cultivated on collagen I, IV, glass, and uncoated plastic dishes. We studied cell proliferation, and expression of ? -SMA, and of alpha, beta, and gamma-crystallins. The effect of medium exchange schedule was studied using the TOTL-86 rabbit epithelial lens cell line. There was no difference in growth characteristics of primary cultures on different substrates. In secondary culture, LECs adhered better on the collagen surfaces. The culture substrate influenced the LEC proliferation and ?-SMA expression. The proliferation was enhanced when the medium was changed in comparison with an extra medium added on the 4th day. The cells did not synthesize ?- or ?-crystalline, the expression of ?-crystallin diminished.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5500

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000265765100006

EID výsledku v databázi Scopus

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